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Effects of transgenes insertion on pollen vigor and hybrid seed set of rice

WEI Xinghua,YUAN Xiaoping,YU Hanyong,WANG Yiping,TANG Shengxiang,LIAO Xiyuan    

  1. State Key Laboratory of Rice Biology,China National Rice Research Institute, Hangzhou 310006,China

  • Received:2003-12-22 Revised:2004-04-10 Online:2005-01-18

Abstract: In this paper,the effects of transgenes insertion on the outcross potentiality of rice varieties were assessed by pollen vigor and hybrid seed set.The in vitro pollen germination rates of five transgenic rice lines transformed respectively with bar,crylAb,BADH and Xa21 gene were investigated,and compared with their relative receptor rice varieties.The results showed that there existed a significant difference in in vitro pollen germination rates between receptor rice varieties,but no significant difference was found between transgenic rice varieties and their relative receptors.The in vitro pollen germination rate for transgenic rice varieties varied from 0.416 to 0.584,similar to that of their relative receptors(0.400~0.574). Investigation on the hybrid seed set of 26 hand-crosses showed that the inserted bar or crylAb gene had a significant effect on the hybrid seed set of receptors,while the effect of Xa21 gene was smaller.The hybrid seed set rate of nontransgenic rice in crosses with transgenic rice(pollen donor)was from 0.056 to 0.413,not different from that in crosses with their relative receptors(0.052~0.417).It's suggested that transgenes insertion had little effect on the outcross potentiality of rice varieties.

Key words: Spodoptera litura, Nuclear polyhedrosis virus, Disease prevalence, Time-dosage-mortality model