Effects of heavy metal ions on SOD activity of Litopenaeus vannamei hepatopancreas,gill and blood

Abstract

Abstract: This paper studied the effects of Cu²⁺,Zn²⁺and Cd²⁺on the superoxide dismutase (SOD) activity of Litopenaeus vannamei hepatopancreas,gill and blood.The results showed that the SOD activity changed significantly with prolonged exposure of these ions (P<0.05).The SOD activity of all test objectives changed with a single peak under the exposure of 0.1～1 mg Cu²⁺·L^-1,that of hepatopancreas and of hepatopancreas and gills was inhibited obviously under 10 mg Zn²⁺·L^-1 and 0.5 mg Cd²⁺·L^-1,respectively,while 0.25 mg Cd²⁺·L^-1 had no significant effect on that of gill.The SOD activity of hepatopancreas,gill and blood all increased first and then decreased under the prolonged exposure of <10 mg Zn²⁺·L^-1and <0.25 mg Cd²⁺·L^-1.There was an obvious dose-time response relationship between test metal ions and SOD activity.The SOD activity was decreased in order of hepatopancreas>gill>blood,while the toxicity of test metal ions was in order of Cd²⁺>Cu²⁺>Zn²⁺.

Key words: Heavy metal ions, Itopenaeus vannamei, Hepatopancreas, Gill, Blood, SOD

CLC Number:

X171.5

WU Zhongwang, PAN Luqing, ZHANG Hongxia . Effects of heavy metal ions on SOD activity of Litopenaeus vannamei hepatopancreas,gill and blood[J]. Chinese Journal of Applied Ecology, 2005, 16(10): 1962-1966.

References

[1] Bagchi D, Vuchetich PJ, Bagchi M, et al. 1998. Protective effect of zinc salts on TPA-induced hepatic and brain lipid peroxidation, glutathionedepletion, DNA damage and peritoneal macrophage activation in mice. General Pharmacol, 30(1): 43～50
[2] Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem, 72: 248～254
[3] Buzadzic B, Korac B, Lazic T, et al. 2002. Effect of supplementation with Cu and Zn on antioxidant enzyme activity in the rat tissues. Food Res Inter, 35:217～220
[4] Dai J-Y(戴家银), Zheng W-Y(郑微云), Wang S-H(王淑红).1998. Effects of copper and zinc ions on activities of enzymes in tissues of red sea bream Pagrosomus major juvenile. Environ Sci (环境科学), 19(5) :60～62(in Chinese)
[5] Dai X-L(戴习林), Zang W-L(臧维玲), Yang H-S(杨鸿山), et al. 2001. The toxic effects of Cu2 +, Zn2 +, Cd2 + on giant freshwater prawn juvenile. J Shanghai Fish Univ(上海水产大学学报), 10(4) :295～302 (in chinese)
[6] Fang Y-Z(方允中), Zheng R-L(郑荣梁). 2002. Theory and Application of Free Radical Biology. Beijing: Science Press. 122～212(in Chinese)
[7] Feng T(冯涛), Zheng W-Y(郑微云), Hong W-S(洪万树), et al. 2001. Effect of benzo(a) pyrene on antioxidant enzyme activities in Boleophthalmus pectinirostris liver. Chin J Appl Ecol (应用生态学报), 12 (3): 422～424 (in Chinese)
[8] Florence G, Angela S, Luisa B, et al. 2002. Response of antioxidant systems to copper in the gills of the clam Ruditapes decussates.Mar Environ Res, 54:413～417
[9] Fridovich I. 1986. Biological effects of superoxide radical. Arch Biochem Biophys, 247:1～11
[10] Halliwell B, Gutteridge JMC. 1989. Free Radicals in Biology and Medicine. Oxford: Clarendon Press. 543
[11] Hou L-P(侯丽萍), Ma G-Z(马广智). 2003. Effects of Cd²⁺on activities of SOD in liver tissue of Ctenopharyngodon idellus. Res Fish (水利渔业), 23(3): 14～15(in Chinese)
[12] Hou L-Y(侯兰英),Zhao H-R(赵鸿儒), Wu Y-L(吴玉霖). 1993.Acute intoxication of some heavy metals to mugil so-iuy and the avoidance reaction of mugil so-iuy. Oceanol Limnol Sin (海洋与湖沼), 24(5): 507～510(in Chinese)
[13] Johnson MA, Fischer JG, Kays SE. 1992. Is copper an antioxidant nutrient? Crit Rev Food Sci Nutr, 32:1～31
[14] Kong F-X(孔繁翔). 2000. Environmental Biology. Beijing: Higher Education Press. 72～74(in Chinese)
[15] Lan W-G(蓝伟光), Wu Y-P(吴永沛), Yang S-K(杨孙楷).1990. Progress of research on toxicity of seawater pollutant to the prawn. Fujian Fish (福建水产), (1) :41～45(in Chinese)
[16] Lan W-G(蓝伟光), Yang S-K(杨孙楷). 1993. Studies on effects of heavy metals on phisiological and biochemical characters of red sea bream Pagrosomus. Acta Ocean Sin (海洋学报), 15(1) :92～97(in Chinese)
[17] Li Y-P(李毅平), Gong H(龚和). 1998. Progress in research on insect antioxidant system. Chin Bull Life Sci (生命科学), 10(5):240～243(in Chinese)
[18] Liang D-H(梁德海), Liu F-Y(刘发义). 1990. Effects of dietary copper on the prawn, Penaeus orientalis. Oceanol Limnol Sin (海洋与湖沼), 21(5) :404 ～410(in Chinese)
[19] Liang D-H(梁德海), Liu F-Y(刘发义), Sun F(孙凤), et al.1989. Effects of dietary zine on the prawn, Penaeus orientalis. Mar Sci (海洋科学),5:49～52(in Chinese)
[20] LiaoL(廖琳),HuX-R(胡晓荣),LiH(李晖),et al.2002.Research progress in Cd toxicity mechanisms and the antagonistic mechanisms of Se to Cd. Sichuan Environ (四川环境), 21 (2): 21～24 (in Chinese)
[21] Livingstone DR. 1993. Biotechnology and pollution monitoring:Use of molecular biomarkers in the aquatic environment. J Chem Technol Biotechnol, 57:195～211
[22] LiuC-F(刘长发),TaoS(陶澍),CaoJ(曹军), et al. 2000.Uptake of particulate lead via gills of gold fish. Chin J Appl Ecol (应用生态学报), 11(2) :283～286(in Chinese)
[23] Liu C-F(刘长发), Tao S(陶澍), Long A-M(龙爱民), et al.2001. Interactions of lead and cadmium on uptake and accumulation by gold fish, Carassius auratus. Acta Ecol Sin (生态学报), 21(11): 1863～1868(in Chinese)
[24] LongA-M(龙爱民),PanB(潘波),Xu F-L(徐福留),et al.2002. Uptake of copper adsorbed on Kaolin of various size by fish (Cyprinus carpio) gills at various pH levels. Acta Ecol Sin (生态学报), 22(10) :1640～1644(in Chinese)
[25] Lu S-Q(鲁双庆), Liu S-J(刘少军), Liu H-Y(刘红玉), et al.2002. Effects of Cu2 + on activities of protecting enzymes SOD, CAT and GSH-PX in liver tissue of Monopterus albus. J Fish Sci China (中国水产科学), 9(2): 138～141 (in Chinese)
[26] Lu X-J(陆星家),Xi Y-L(席贻龙), Liu G-Y(刘桂云).2004.Accumulation and distribution of lead in Macrobrachium nipponense.Chin J Appl Ecol(应用生态学报), 15(1): 131～135(in Chinese)
[27] Pan L-Q(潘鲁青), Ren J-Y(任加云), Wu Z-W(吴众望).2004.Effects of heavy metal ions on SOD, CAT activities of hepatopancreas and gill on the crab Eriocheir sinensis. J Ocean Univ China (中国海洋大学学报), 34(2): 189～194(in Chinese)
[28] Parke VD. 1987. Role of enzymes in protection against lipid peroxidation. Regul Toxicol Pharmacol, 7:222～235
[29] Rainbow PS. 1992. The heavy metal accumulation and its meaning in marine organisms. Mar Environ Sci(海洋环境科学), 11 (1): 44～51 (in Chinese)
[30] Roche H, Bogé G. 1996. Fish Blood Parameters as a Potential Tool for Identification of Stress Caused by Environmental Factors and Chemical Intoxication. Mar Environ Res,41 (1): 27～43
[31] Siraj BP, Usha RA. 2003. Cadmium-induced antioxidant defense mechanism in freshwater teleost Oreochromis mossambicus(Tilapia). Ecotoxicol Environ Safety, 56: 218～221
[32] Stebbing ARD. 1982. Hormesis the stimulation of growth by low levels of inhibition. Sci Tol Environ, 22 (1): 213～234
[33] Stem A. 1985. Red cell oxidative damage. In: Sites H. ed. Oxidative Stress. New York:Academic Press. 331～349
[34] WangS-B(王寿兵),GuoR(郭锐),QuY-F(屈云芳),et al.1998. Acute toxicity of Cu2 + to Rana chensinensis tadpole. Chin J Appl Ecol (应用生态学报), 9 (3) : 309～312 (in Chinese)
[35] Winston GW. 1991. Oxidants and antioxidants in aquatic animals.Comp Biochem Physiol, 100C(1/2): 173～176
[36] Wiston GW, Di Giulio T. 1991. Prooxidant and antioxidant mechanisms in aquatic organisms. Aquat Toxicol, 19:137～161
[37] Wu J (吴坚). 1992. Biochemical effects of trace metals on marine animals. Mar Environ Sci (海洋环境科学), 10 (2): 58～64 (in Chinese)
[38] Xu L-H(徐立红),Zhang Y-Y(张甬元), Chen Y-Y(陈宜瑜).1995. The advances of molecular ecotoxicology and its significance in water environment protection. Acta Hydrobiol Sin (水生生物学报), 19(2): 171～185(in Chinese)
[39] Xu L, Zheng GJ, Lam PKS, et al. 1999. Relationship between tissue concentrations of polycyclic aromatic hydrocarbons and DNA adducts in green-lipped mussels(Perna viridis). Ecotoxicology, 8:73～82
[40] Yang L-H(杨丽华), Fang Z-Q(方展强), Zheng W-B(郑文彪), et al. 2003. Experiment with effect of cadmium on activity of superoxide dismutase in gill and liver tissue of crucian. J Safety Environ (安全与环境学报), 3(3): 13～16(in Chinese)
[41] Yang L-H(杨丽华), Fang Z-Q(方展强), Zheng W-B(郑文彪).2003. Safety assessment and acute toxicity of heavy metals to crucian Carassius auratus. J South China Normal Univ (Nat Sci) (华南师范大学学报·自然科学版), (2): 101～106(in Chinese)
[42] Zhao H-X(赵红霞),Zhan Y(詹勇),Xu Z-R(许梓荣).2003.Progress in studying the toxic effects of heavy metals on aqutic animals. Inland Aquatic Product (内陆水产), 28 (1): 38～40 (in Chinese)
[43] Zou G-L(邹国林), Hu W-Y(胡文玉), Qiu T(邱涛), et al.1997. Studies on the sensitivity of some methods for activity assay of superoxide dismutase. J Wuhan Univ(Nat Sci) (武汉大学学报·自然科学版), 43(2): 233～237(in Chinese)