Bacterial flora composition and its dynamics in tidal-flat Sinonovacula constricta aquaculture area

Abstract

Abstract: In this study,the bacteria from the mud in tidal-flat Sinonovacula constricta aquaculture area were isolated each month from March to December,2002,and the temporal and spatial distribution of heterotrophic bacteria,ammonifying bacteria,denitrifying bacteria,and sulphate reducing bacteria were analyzed.The results showed that all the 515 isolated bacteria mainly belonged to 1 family and 13 genera.The bacterial flora in different layers of the mud was almost consistent,while the composition was different.The predominant genera were Clostridium,Bacillus,Corynebacterium,Photobacterium,and some Enterobacteriaceae.The number of heterotrophic bacteria in the surface layer and the bottom fluctuated in 7.6×10³ cfu·g^-1～2.0×10⁵ and 1.6×10³～1.0×10⁵ cfu·g^-1,ammonifying bacteria fluctuated in 1.5×10⁶～9.0×10⁷ and 9.0×10⁵～1.0×10⁷ cfu·g^-1,denitrifying bacteria fluctuated in 9.0×10³～4.0×10⁶ and 5.0×10²～1.9×10⁶ cfu·g^-1,and sulphate reducing bacteria fluctuated in 5.0×10⁴～5.0×10⁶ and 1.9×10⁴～2.0×10⁶ cfu·g^-1,respectively.The detection rates of ammonifying bacteria,denitrifying bacteria and sulphate reducing bacteria in the mud were all 100%,and these bacteria increased significantly in the second half of the year,indicating that the environment of the Sinonovacula constricta aquaculture area was deteriorated due to the accumulation of NH₃,nitrite and H₂S,and it is important to regulate the breed capacity and redistribute the breeding environment.

Key words: Sinonovacula constricta, Tidal-flat area, Heterotrophic bacteria, Ammonifying bacteria, Denitrifying bacteria, Sulphate reducing bacteria

CLC Number:

JIN Shan, XUE Chaobo, WANG Guoliang, LU Tongxia, WANG Yinong, CHEN Yiner . Bacterial flora composition and its dynamics in tidal-flat Sinonovacula constricta aquaculture area[J]. Chinese Journal of Applied Ecology, 2005, 16(7): 1380-1382.

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