Abstract: Due to the production,death,and decomposition of fine root,its turnover plays an important role in carbon allocation and nutrient cycling in terrestrial ecosystems.Some methods such as sequential root coring,compartmental flow model,and ingrowth core have been widely used in collecting root biomass data and estimating fine root turnover,but failed in monitoring the dynamics of fine root due to its simultaneous production and death.Minirhizotron is a nondestructive in situ method for studying the dynamics of fine root,which allows the simultaneous measurement of fine root growth and mortality.This paper reviewed the application of minirhizotron in fine root studies,with the focus on minirhizotron tube installation,image collection,data extraction,and calculation parameters.In a case study,the total fine root length,fine root length density per unit volume,fine root length density per unit area,fine root biomass density,and fine root production and mortality of Fraxinus mandshurica and Larix gmelini were calculated,and the results showed that minirhizotron method was feasible in studying the processes of fine root development,eclipse,death,and decomposition.The factors affecting fine root measurement and its precision mainly included the quality and quantity of tube installation,sampling interval and quantity,and analysis technique of images,etc.Soil texture,tube material,and disturbance of light on root were also the factors affecting the precision of the method.How to improve the measurement precision of minirhizotron would be the critical problem in future study.

Key words: Biomarkers, ChE, GST, Gammarus pulex L