Genetic diversity of arbuscular mycorrhizal fungi associated with four Alnus species in Changbai Mountains: A nested PCR-RFLP analysis

Abstract

Abstract: In this paper, the colonization of arbuscular mycorrhizal fungi (AMF) on the root samples of 4 Alnus species in Changbai Mountains was investigated by using nested PCR-RFLP technique, and the PCR results were tested by restriction endonuclease analysis method. The results revealed that the uncultured AMF had a high genetic diversity, and the colonization had a trend from promiscuity to specialization. The AMF from Alnus mandshuica showed the greatest specialization to the host, and Glomus was the dominant colonizer of A. mandshuica. The AMF from A. sibirica var. hirsuta, A. sibirica, and A. tinctoria showed promiscuity, and host had more significant effects on the colonization of AMF than altitude.

Key words: Copper mine tailings, Legumes, Seed germination, Seedling growth, Inhibition

DONG Tian^1,2; ZHANG Huiwen¹;ZHANG Yue¹; HE Xingyuan¹ . Genetic diversity of arbuscular mycorrhizal fungi associated with four Alnus species in Changbai Mountains: A nested PCR-RFLP analysis[J]. Chinese Journal of Applied Ecology.

[1]	GUO Linlin, WANG Jingjing, ZU Jingmei, WANG Pinsu, YANG Yujie. Effects of microplastics on seed germination and seedling physiological characteristics of Spinacia oleracea under alkali stress [J]. Chinese Journal of Applied Ecology, 2023, 34(9): 2536-2544.
[2]	WEN Lilian, SONG Jinming, LI Xuegang, MA Jun, DAI Jiajia, YUAN Huamao, DUAN Liqin, WANG Qidong. Environmental pollution of fluoroquinolones and its relationship with nitrogen cycling mediated by microorganisms. [J]. Chinese Journal of Applied Ecology, 2023, 34(11): 3114-3126.
[3]	ZHAO Hai-liang, ZUO Lu, ZHANG Lu, GUO Tian-yu, ZHANG Yi, LI Xiao-jing, HU Xiao-hui, WANG Yu-ping. Mitigation of exogenous melatonin on photoinhibition of tomato seedlings under chilling stress [J]. Chinese Journal of Applied Ecology, 2023, 34(1): 151-159.
[4]	GOU Qian-qian, GAO Min, ZHANG Yu, WANG Guo-hua. Morphology of Caragana korshinskii seeds across different stand ages in sandy-hilly region of northwest Shanxi Province, China. [J]. Chinese Journal of Applied Ecology, 2022, 33(11): 2907-2914.
[5]	LI Xue-hong, LI Dong-po, WU Zhi-jie, CUI Lei, XIAO Fu-rong, LI Yong-hua, ZHENG Ye, ZHANG Jin-ming. Effect of urease/nitrification inhibitor on urea nitrogen conversion in black soil and cinnamon soil. [J]. Chinese Journal of Applied Ecology, 2021, 32(4): 1352-1360.
[6]	LIU Lei, XU Meng, ZHANG Guo-yin, WANG Ling, SUN Shi-you, RU Shu-hua, XIAO Guang-min, GAO Jing, LI Pin, MA Li-min. Seasonal variations of arbuscular mycorrhizal fungal community in greenhouse soil under different rotation systems [J]. Chinese Journal of Applied Ecology, 2021, 32(11): 4095-4106.
[7]	WANG Xiu-qing, WANG Jin-xin, MA Xu, ZHANG Yu-yu, WANG Huai-biao, WANG Yu-bao, DI Long. Effects of leaf extracts of Amorpha fruticosa on seed germination and seedling growth of Amygdalus pedunculata [J]. Chinese Journal of Applied Ecology, 2021, 32(1): 57-65.
[8]	PAN Dong-yun, FU Xin, ZHANG Xiao-wei, LIU Feng-jiao, BI Huan-gai, AI Xi-zhen. Hydrogen sulfide acted as a downstream signal was involved in the regulation of salicylic acid on photosynthesis of cucumber seedlings under low temperature and low light intensity [J]. Chinese Journal of Applied Ecology, 2020, 31(9): 3023-3032.
[9]	LI Jin-xin, YE Jun-wei, LIU Da-hui. Allelopathic effects of Miscanthus floridulus on seed germination and seedling growth of three crops [J]. Chinese Journal of Applied Ecology, 2020, 31(7): 2219-2226.
[10]	DONG Shu-qi, CAO Peng, HU Chun-yan, SHER Alam, YUAN Xiang-yang, YANG Xue-fang, GUO Ping-yi. Allelopathic effects of water extracts from different parts of foxtail millet straw on three kinds of weeds [J]. Chinese Journal of Applied Ecology, 2020, 31(7): 2243-2250.
[11]	HANG Wei, GE Yu-ying, LIU Hao-yu, JIANG Hao-ran, ZHANG Shi-hang, TAO Ye. Effects of Brachythecium plumosum and Plagiomnium venustum on seed germination and seedling growth of invasive plants [J]. Chinese Journal of Applied Ecology, 2020, 31(7): 2271-2278.
[12]	GAO Yu-lian, LI Rui-guang, CHANG Jing, LI Yan, LI Hai-ping. Allelopathy of rape on seed germination and seedling growth of three crops [J]. Chinese Journal of Applied Ecology, 2020, 31(12): 4153-4160.
[13]	YAN Xing-fu, DENG Xiao-juan, WANG Jing, ZHOU Li-biao, ZHANG Jin-feng, LUO Yong-hong. Effects of seed size and drought stress on the growth and physiological characteristics of Quercus wutaishanica seedlings [J]. Chinese Journal of Applied Ecology, 2020, 31(10): 3331-3339.
[14]	SUN Jin-wei, YAO Fu-qi, ZHANG Zhen-hua. Differences of leaf dark respiration and light inhibition between saplings and mature trees of Pinus koraiensis and Tilia amurensis. [J]. Chinese Journal of Applied Ecology, 2019, 30(5): 1454-1468.
[15]	LI Cui, JING Ju-hui, LIU Jin-xian, CHAI Bao-feng. Spatiotemporal dynamics and driving forces of soil bacterial communities on the dam of Shibahe copper mine tailings in Shanxi, China. [J]. Chinese Journal of Applied Ecology, 2018, 29(6): 1975-1982.

Genetic diversity of arbuscular mycorrhizal fungi associated with four Alnus species in Changbai Mountains: A nested PCR-RFLP analysis

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments