Abstract: The study on the heavy metals-polluted soil of Qingshuitang District, Zhuzhou City showed that the main enriched heavy metals in the soil were Cd, Hg, As, Zn,Pb and Cu, among which, the former three had strong biotoxicity while the others had definite toxicity, with the average integrative pollution index being 6.40. The heavy metals enrichment in soil animals increased with increasing pollution degree. Especially for some species of Megascoiecidae, they had a high enrichment of Cd, with the enrichment index being 11.96. The species and quantity of soil animals decreased with increasing Cd concentration. Cd had an obvious biotoxic effect on Megsacoiecidae’s isozyme activity. The heavy metals enrichment in vegetables also increased with increasing pollution degree, but there existed obvious interspecies difference in Cd enrichment.