Chinese Journal of Applied Ecology ›› 2010, Vol. 21 ›› Issue (01): 203-208.
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LAN Yi-quan, ZHAO Shi-xi
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Abstract: By using synergist bioassay and biochemical analysis, this paper approached the resistance mechanisms of Spodoptera exigua (Hübner) to fenvalerate and alpha-cypermethrin. The synergistic ratios of piperonyl butoxide (PBO), o,o-diethyl-o-phenyl-thiophosphate (SV1), triphenyl phosphate (TPP), and diethyl maleate (DEM) between fenvalerate-resistant strain (Fen-R) and susceptible strain were 10.2, 7.8, 12.5, and 1.1, and those between alpha-cypermethrin resistant strain (Cyp-R) and susceptible strain were 21.6, 15.5, 8.6, and 1.2, respectively. Significant synergisms of PBO, SV1, and TPP to fenvalerateand alpha-cypermethrin wereobserved, implying that multifunctional oxidase and carboxylesterase were involved in the resistance to fenvalerate and alpha-cypermethrin. The carboxylesterase activities in the fourth instar larvae of Cyp-Rand Fen-R strains were 1.9 and 2.2 folds of the corresponding susceptible strains, respectively, but no differences were found in the glutathione-S-transferase activities between the resistant and susceptible strains, which indicated that carboxylesterase played an important role in the resistance of S. exigua to fenvalerate and alpha-cypermethrin, while glutathione-S-transferase contributed little to the resistance. There were no significant differences in the Na-K-ATPase activities between the resistant and susceptible strains, but the inhibition of fenvalerate and alpha-cypermethrin on Na-K-ATPase was higher in the susceptible strains than in the resistant strains, indicating the decreased sensitivity of Na-K-ATPase in resistant strains.
Key words: Spodoptera exigua, fenvalerate, alpha-cypermethrin, insecticide resistance, biochemical mechanism, socio-hydrology, coupled human-water system, sustainable utilization of water resources, two-way feedbacks.
LAN Yi-Quan, ZHAO Shi-Xi. Resistance mechanisms of Spodoptera exigua (Hübner) to fenvalerate and alpha-cypermethrin.[J]. Chinese Journal of Applied Ecology, 2010, 21(01): 203-208.
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URL: https://www.cjae.net/EN/abstract/abstract3529.shtml
https://www.cjae.net/EN/Y2010/V21/I01/203