Abstract: This paper studied the effects of different storage medium (coelomic fluid, Hepes solution, and modified Ringer’s solution), temperature (4 ℃ and 16℃), and duration (4 h, 8 h, 16 h, and 24 h) on the short-term storage of Siberian sturgeon (Acipenser baerii) eggs. The results showed that storage medium, temperature, and duration all had significant effects on the fertilization rate, hatching success, and malformation of newly hatched larvae (P<0.05). With the increase of storage duration, the fertilization and hatching rates decreased, while the malformation rate of hatched larvae increased. The A. baerii eggs stored at 16 ℃ had higher fertilization, hatching, and malformation rates but shorter holding time than those stored at 4 ℃. The optimal conditions for the short-term storage of A. baerii  eggs were Hepes solution prepared according to the biochemical composition of A. baerii coelomic fluid, 16 ℃, and 4 h, under which, 86.36% fertilization rate, 94.74% hatching rate, and zero malformation would be obtained.

Key words: Acipenser baerii, egg, storage medium, temperature, short-term storage, ecosystem services, urban ecology, landscape pattern, assessment, tradeoff.