Abstract: A pot experiment was conducted to study the microbial activity and community structure in a lead-polluted paddy soil under effects of amendment with
different concentration sodium thiosulfate. The amendment of the sulfur-containing substrate increased the soil oxidation-reduction potential and respiration rate, promoted the growth of soil sulfur-oxidizing bacteria, and induced some changes in soil microbial community structure. The clone sequencing indicated that the specific bands of soil microbes in sodium thiosulfate treatments had very high similarity to Bateroidetes, Thiobacillus, β-proteobacteria, and Acidobacteria. Significant changes were observed in the contents of soil carbonate- and Fe/Mn oxide- bound Pb after the amendment of sodium thiosulfate.

Key words: paddy soil, lead, sulfur, microbial community, sulfur-oxidizing bacteria, Guiyang City, urban expansion, space simulation, optimal path.