Abstract: This study estimated the spatial distribution of the aboveground biomass of shrubs in the Tianlaochi catchment of Qilian Mountains based on the field survey and remote sensing data. A relationship model of the aboveground biomass and its feasibly measured factors (i.e., canopy perimeter and plant height) was built. The land use was classified by objectoriented technique with the high resolution image (GeoEye-1) of the study area, and the distribution of shrub coverage was extracted. Then the total aboveground biomass of shrubs in the study area was estimated by the relationship model with the distribution of shrub coverage. The results showed that the aboveground biomass of shrubs in the study area was 1.8×10³ t and the aboveground biomass per unit area was 1598.45 kg·m^-2. The distribution of shrubs mainly was at altitudes of 3000-3700 m, and the aboveground biomass of shrubs on the sunny slope (1.15×10³ t) was higher than that on the shady slope (0.65×10³ t).