Abstract: Decomposition of soil organic matter plays an important role in the regulation of carbon (C) cycles at ecosystem or regional scales, and is closely related to temperature, moisture, and landuse types. The influences of soil temperature, moisture, and landuse types on soil C mineralization in Citrus reticulata and Pinus elliottii forests were investigated at the Qianyanzhou Ecological Experiment Station, Chinese Academy of Sciences, by conducting incubation experiments at 5-level temperatures (5, 10, 15, 20 and 25 ℃) and 3-level moistures (30%, 60% and 90% saturated soil moisture, SSM). The results showed that soil temperature, moisture, and land-use types had significant effects on soil C mineralization and they had significant interaction effects. Soil C mineralization was positively correlated with incubation temperature in the two forests, and the maximum of soil C mineralization was in the 60% SSM treatment. The accumulation of soil C mineralization was higher in the C. reticulata forest than in the P. elliottii forest under the same temperature and moisture conditions. The temperature sensitivity (Q₁₀) of soil C mineralization was influenced by landuse type and soil moisture. Q₁₀ increased with the increasing soil moisture in both C. reticulata and P. elliottii forests at incubation 7 and 42 d. Q₁₀ in the C. reticulata forest was higher than in the P. elliottii forest in the same moisture level, and the deviation increased with the increasing soil moisture. The model including temperature and moisture could depict the response of soil C mineralization to temperature and moisture. Temperature and moisture together explained 79.9%-91.9% of the variation in soil C mineralization.