Effects of elevated ozone concentrations on enzyme activities and organic acids content in wheat rhizospheric soil.

doi:10.13287/j.1001-9332.201802.022

Abstract

Abstract: The elevated concentration of tropospheric ozone (O₃) is an important global climate change driver, with adverse impacts on soil ecological environment and crop growth. In this study, a pot experiment was carried out in an open top chamber (OTC), to investigate the effects of elevated ozone concentration on soil enzyme activities (catalase, polyphenol oxidase, dehydrogenase and invertase), organic acids contents (oxalic acid, citric acid and malic acid) at different growth stages (tillering, jointing, heading and ripening stages) of wheat, and combined with the rhizospheric soil physicochemical properties and plant root characteristics to analyze the underlying reasons. The results showed that, elevated ozone concentration increased soil catalase, polyphenol oxidase, dehydrogenase and invertase activities at wheat ripening period to different degrees, with the effects on the activities of catalase and polyphenol oxidase being statistically significant. At the heading stage, activities of dehydrogenase and invertase were significantly increased by up to 76.7%. At the ripening stage, elevated ozone concentration significantly increased the content of citric acid and malic acid and redox potential (Eh) in rhizospheric soil, but reduced soil pH, electrical conductivity, total carbon and nitrogen. For root characteristics, elevated ozone concentrations significantly reduced the wheat root biomass, total root length and root surface area but increased the average root diameter.

YIN Wei-qin, JING Hao-qi, WANG Ya-bo, WEI Si-yu, SUN Yue, WANG Sheng-sen, WANG Xiao-zhisup>1,2*. Effects of elevated ozone concentrations on enzyme activities and organic acids content in wheat rhizospheric soil.[J]. Chinese Journal of Applied Ecology, 2018, 29(2): 547-553.

References

[1] Karlsson PE, Klingberg J, Engardt M, et al. Past, present and future concentrations of ground-level ozone and potential impacts on ecosystems and human health in northern Europe. Science of the Total Environment, 2016, 576: 22-35
[2] Ashmore M, Emberson L, Karlsson PE, et al. Introduction for ozone deposition special issue. Atmospheric Environment, 2004, 38: 2211-2212
[3] Vingarzan R. A review of surface ozone background levels and trends. Atmospheric Environment, 2004, 38: 3431-3442
[4] IPCC. Climate Change 2007: The Physical Science Basis: Contribution of Working Group Ⅰ to the Fowth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge: Cambridge University Press, 2007: 1-996
[5] Sui L-H (隋立华), Huang Y-Z (黄益宗), Wang W (王玮), et al. Effects of elevated ozone on antioxidant system of winter wheat leaf in varied growth stages. Asian Journal of Ecotoxicology (生态毒理学报), 2011, 6(5): 507-514 (in Chinese)
[6] Zheng Y-F (郑有飞), Hu C-D (胡程达), Wu R-J (吴荣军), et al. Experiment with effects of increased surface ozone concentration upon winter wheat photosynthesis. Acta Ecologica Sinica (生态学报), 2010, 30(4): 847-856 (in Chinese)
[7] Liang J (梁晶), Zeng Q (曾青), Zhu J-G (朱建国), et al. Effects of O₃-FACE (Ozone-free Air Control Enrichment) on respiration enzymes of rice leaf. Chinese Agricultural Science Bulletin (中国农学通报), 2010, 26(6): 260-264 (in Chinese)
[8] Yu Y-C (余永昌), Lin X-G (林先贵), Zhang J (张晶), et al. Effect of elevated surface O₃ concentration on soil microbial functional diversity in wheat field. Chinese Journal of Applied and Environmental Biology (应用与环境生物学报), 2012, 18(2): 309-314 (in Chinese)
[9] Wang J-S (王劲松), Fan F-F (樊芳芳), Guo J (郭珺), et al. Effect of different crop rotations on growth of continuous cropping sorghum and its rhizosphere soil micro-environment. Chinese Journal of Applied Ecology (应用生态学报), 2016, 27(7): 2283-2291 (in Chinese)
[10] Ning C-C (宁川川), Wang J-W (王建武), Cai K-Z (蔡昆争). The effects of organic fertilizers on soil fertility and soil environmental quality: A review. Ecology and Environmental Sciences (生态环境学报), 2016, 25(1): 175-181 (in Chinese)
[11] Yu Y-C (余永昌), Lin X-G (林先贵), Feng Y-Z (冯有智), et al. Effects of elevated surface O₃ concentration on soil biological properties in a rice/wheat rotation field. Soils (土壤), 2012, 44(3): 450-455 (in Chinese)
[12] Yang R (杨瑞), Liu S (刘帅), Wang Z-Q (王紫泉), et al. Relationships between the soil enzyme activity and soil nutrients in forest soils typical of the Qinling Mountain. Acta Pedologica Sinica (土壤学报), 2016, 53(4): 1037-1046 (in Chinese)
[13] Zhang Y (张勇), Chen S-T (陈书涛), Wang L-X (王连喜), et al. Effects of elevated ozone concentration on soil respiration, nitrification and denitrification in a winter wheat farmland. Environmental Science (环境科学), 2010, 31(12): 2988-2994 (in Chinese)
[14] Huang W-B (黄文斌), Ma R (马瑞), Yang D (杨迪), et al. Organic acids secreted from plant roots under soil stress and their effects on plant ecological adaptability. Journal of Anhui Agricultural Sciences (安徽农业科学), 2013, 41(34): 13316-13319 (in Chinese)
[15] Li C-Y (李春艳), Li C-R (李传荣), Xu J-W (许景伟), et al. Study on soil nutrients, microbes and enzyme activities of different stands of silting coastal area. Journal of Soil and Water Conservation (水土保持学报), 2007, 21(1): 156-159 (in Chinese)
[16] Shi C-E (施翠娥), Ai F-X (艾弗逊), Wang C-R (汪承润), et al. Effects of elevated atmospheric CO₂ and O₃ on soil enzyme activities and microbial biomass. Journal of Agro-Environment Science (农业环境科学学报), 2016, 35(6): 1103-1109 (in Chinese)
[17] Wang X, Agathokleous E, Qu L, et al. Effects of CO₂ and O₃ on the interaction between root of woody plants and ectomycorrhizae. Journal of Agricultural Meteorology, 2016, 72: 95-105
[18] Bao S-D (鲍士旦). Soil and Agrochemical Analysis. Beijing: China Agriculture Press, 1988 (in Chinese)
[19] Cai K-Z (蔡昆争), Luo S-M (骆世明), Duan S-S (段舜山). The response of rice root system to different light intensity after root confinement. Journal of South China Agricultural University (华南农业大学学报), 2007, 28(1): 1-5 (in Chinese)
[20] Zhou L-K (周礼恺), Zhang Z-M (张志明). The determination methods of soil enzyme activity. Chinese Journal of Soil Science (土壤通报), 1980, 11(5): 38 (in Chinese)
[21] Wang P (王平), Zhou R (周荣). Determination of organic acids exuded from plant roots by high performance liquid chromatography. Chinese Journal of Chromatography (色谱), 2006, 24(3): 239-242 (in Chinese)
[22] Li R (李锐), Liu Y (刘瑜), Chu G-X (褚贵新). Effects of different cropping patterns on soil enzyme activities and soil microbial community diversity in oasis farmland. Chinese Journal of Applied Ecology (应用生态学报), 2015, 26(2): 490-496 (in Chinese)
[23] Geng C-M (耿春梅), Wang Z-S (王宗爽), Ren L-H (任丽红), et al. Study on the impact of elevated atmospheric ozone on crop yield. Research of Environmental Sciences (环境科学研究), 2014, 27(3): 239-245 (in Chinese)
[24] Jing L, Dombinov V, Shen S, et al. Physiological and genotype-specific factors associated with grain quality changes in rice exposed to high ozone. Environmental Pollution, 2016, 210: 397-408
[25] Feng ZZ, Kazuhiko K, Elizabetha A. Impact of elevated ozone concentration on growth, physiology, and yield of wheat (Triticum aestivum L.): A meta-analysis. Global Change Biology, 2008, 14: 2696-2708
[26] Chen Z (陈展), Wang X-K (王效科), Feng Z-Z (冯兆忠), et al. Effects of elevated ambient ozone on ecosystem below ground processes. Chinese Journal of Ecology (生态学杂志), 2007, 26(1): 121-125 (in Chinese)
[27] Xu Z-F (徐振锋), Tang Z (唐正), Wan C (万川), et al. Effects of simulated warming on soil enzyme activities in two subalpine coniferous forests in west Sichuan. Chinese Journal of Applied Ecology (应用生态学报), 2010, 21(11): 2727-2733 (in Chinese)
[28] Huang Y-Z (黄益宗), Wang F (王斐), Zhong M (钟敏), et al. Effects of elevated ozone on carbon, nitrogen content and soil enzymes activities in a winter wheat field. Asian Journal of Ecotoxicology (生态毒理学报), 2013, 8(6): 871-878 (in Chinese)
[29] Li G-M (李果梅), Wang S-Y (王殳屹), Shi Y (史奕), et al. Effects of elevated ozone and temperature on soil enzymes activities and phenolic compounds content in spring wheat. Journal of Agro-Environment Science (农业环境科学学报), 2008, 27(1): 121-125 (in Chinese)
[30] Jin C-X (金彩霞), Zhu W-F (朱雯斐), Li M-L (李明亮), et al. The dynamic change of organic acid content in the rhizopheric soils. Journal of Arid Land Resources and Environment (干旱区资源与环境), 2013, 27(11): 86-91 (in Chinese)
[31] Wang S-G (王曙光), Feng Z-Z (冯兆忠), Wang X-K (王效科), et al. Effects of elevated atmospheric O₃on arbuscular mycorrhizal (AM) and its function. Environmental Science (环境科学), 2006, 27(9): 1872-1877 (in Chinese)
[32] Geng G (耿贵). Effect of Crop Root Exudates on Carbon and Nitrogen Contents, Microorganisms Quatity and Enzyme Activities in Soil. PhD Thesis. Shengyang: Shengyang Agricultural University, 2011 (in Chinese)
[33] Wu R, Zheng Y, Hu C. Evaluation of the chronic effects of ozone on biomass loss of winter wheat based on ozone flux-response relationship with dynamical flux thres-holds. Atmospheric Environment, 2016, 142: 93-103
[34] Yi L-P (弋良朋), Zhang H (张辉). Characteristics of soil enzymatic activities and relationship with the main nutrient in the rhizosphere of four littoral halophytes. Ecology and Environmental Science (生态环境学报), 2011, 20(2): 270-275 (in Chinese)
[35] Qiu L-P (邱莉萍), Liu J (刘军), Wang Y-Q (王益权), et al. Research on relationship between soil enzyme activities and soil fertility. Plant Nutrition and Fertilizer Science (植物营养与肥料学报), 2004, 10(3): 277-280 (in Chinese)
[36] Kou TJ, Cheng XH, Zhu JG, et al. The influence of ozone pollution on CO₂, CH₄, and N₂O emissions from a Chinese subtropical rice-wheat rotation system under free-air O₃ exposure. Agriculture, Ecosystems & Environment, 2015, 204: 72-81
[37] Lu W-L (陆文龙), Cao Y-P (曹一平), Zhang F-S (张福锁). Role of root exuded organic acids in mobilization of soil phosphorus and micronutrients. Chinese Journal of Applied Ecology (应用生态学报), 1999, 10(3): 379-382 (in Chinese)