Abstract: By using a freeair controlled enrichment (FACE) system, this paper studied the effects of elevated atmospheric ozone (O₃) concentration (150% of ambient O₃) on the flag leaf photosynthetic pigment contents of wheat varieties Yannong 19, Yangmai 16, Jiaxin 002, Yangmai 15, and Yangfumai 2. For the test varieties, no significant differences were observed in the flag leaf chlorophyll a, chlorophyll b, chlorophyll (a+b), and carotenoid contents between treatments elevated O₃ concentration and ambient O₃ at booting and anthesis stages, but the photosynthetic pigment contents in treatment elevated O₃ concentration all decreased after anthesis, with a significant decrease of chlorophyll a, chlorophyll b, and chlorophyll (a+b) contents, which indicated that elevated O₃ had minor effects on the synthesis of photosynthetic pigments but accelerated their decline process. Different wheat varieties had genetic difference in the responses of flag leaf photosynthetic pigment contents to elevated O₃, among which, Yangmai 15 and Jiaxin 002 had better tolerance to ozone stress. The flag leaf chlorophyll a, chlorophyll b, and chlorophyll (a+b) contents at grain-filling stage (about 21 days after anthesis) had a significant positive correlation with the 1000grain mass.