Abstract: Honeybee foragers can flexibly adjust their out-hive activities to ensure growth and reproduction of the colony. In order to explore the characteristics of honey bees foraging behaviors, in this study, their flight activities were monitored 24 hours per day for a duration of 38 days, using an radio frequency identification (RFID) system designed and manufactured by the Honeybee Research Institute of Jiangxi Agricultural University in cooperation with the Guangzhou Invengo Information Technology Co., Ltd. Our results indicated that 63.4% and 64.5% of foragers were found rotating more than one day off during the foraging period in two colonies, and 22.5% and 26.4% of the total foraging days were used for rest respectively. Further, although the total foraging time between rotating dayoff foragers and continuously working foragers was equal, the former had a significant longer lifespan than the latter. Additionally, the lifespan of the early developed foragers was significantly lower than that of the normally developed foragers. This study enriched the content of foraging behaviors of honey bees, and it could be used as the basis for the further explorations on evolutionary mechanism of foraging behaviors of eusocial insects.