Abstract: The lysosome of Eisenia fetida can accumulate neutral red dye rapidly.When exposed to environment pollution,the membrane of the lysosome is easily to be cracked,and the dye shall leak to the cytoplasm gradually,making the cell red.Therefore,neutral red retention time (NRRT) can reflect the damaged degree of lysosome.In this paper,the NRRT of the lysosome in coelomocytes of E.fetida was determined after exposed to different concentrations of acetochlor and selenium.The results showed that with increasing exposure time and increasing concentration of acetochlor and Se,the NRRT was decreased significantly,and had significant correlations with the concentrations of acetochlor in soil and of accumulated Se in E.fetida’s body.NRRT could be served as a biomarker of soil pollution,and a promising tool for the early warning of potential danger.

Key words: Nerium indicum, Oncumelania hupensis, Killing effect