Abstract: The effects of three canopy shapes, i.e., vertical canopy, V shaped canopy and horizontal canopy, on canopy microenvironment, quality of leaves and fruits were studied in the 3-year-old grape ‘Jingmi’ grafted on ‘Beta’ in greenhouse. The results showed that gap fraction and openness of vertical canopy were significantly higher than that of Vshaped canopy and horizontal canopy, and leaf area index, light interception rate and canopy temperature difference between day and night were significantly lower than those of V shaped canopy and horizontal canopy. There was no significant difference between the latter two treatments. The palisade thickness of V-shaped canopy was significantly greater than that of vertical canopy, and horizontal canopy was in the middle. The chlorophyll and carotenoid contents of V shaped canopy were significantly higher than those of vertical canopy and horizontal canopy, and those in the latter two treatments had no significant difference. The fruit quality of V shaped canopy was the best, and that of horizontal canopy was the worst. The results of GC-MS analysis showed that 29 types of volatile aroma compounds were detected in V-shaped canopy, but just 17 and 16 in vertical canopy and horizontal canopy, respectively. In V shaped canopy, the characteristic aroma in grape ‘Jingmi’ was higher, except ethanol, trans-2-hexene-1-alcohol, 2-octyl ketone and formic acid ester. The linalool content in vertical canopy and V shaped canopy was higher than that in horizontal canopy. The nerol content in Vshaped canopy was higher than that in vertical canopy and horizontal canopy, and the leaf alcohol content in Vshaped canopy and horizontal canopy was higher than that in vertical canopy. The citronellol was detected only in Vshaped canopy. In greenhouse, the fruit aroma of Vshaped canopy grape was stronger, and well reflected the variety characteristics.