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Effects of NO3-stress and recovery on chlorophyll fluorescence parameters and ATPase activities of cucumber seedling leaves

YANG Fengjuan1;WANG Xiufeng1;WEI Min1;SU Xiurong2;YAN Tong1   

  1. 1College of Horticulture Science and Engineering,Shandong Agricultural Univeristy,Tai'an 271018,China;2College of Chemistry and Material Science,Shandong Agricultural University,Tai’an 271018,China

  • Received:2005-04-01 Revised:2005-07-11 Online:2006-03-18 Published:2006-03-18

Abstract: With water culture,this paper studied the effects of NO3- stress and recovery on the chlorophyll contents,chlorophyll fluorescence parameters,and ATPase activities of cucumber seedling leaves.The results showed that under stress for 7 days,the chlorophyll a,chlorophyll b,total chlorophyll and carotenoid contents,intrinsic fluorescence (Fo),and MgATPase and Ca-ATPase activities were increased significantly when the NO3- concentration was high (168 mmol ·L-1),while the intrinsic photochemical efficiency (Fv/Fm),potential activities (Fv/Fo) and quantum yield (ΦPSⅡ) of PSⅡ were decreased with increasing NO3- concentration.After recovery for 7 days,the chlorophyll and carotenoid contents of all treatments with NO3- were lower than CK,while the intrinsic fluorescence,intrinsic photochemical efficiency and quantum yield of PSⅡ were similar to CK when the NO3- concentration was ≤ 84 mmol·L-1,and the Fv/Fm and ΦPSⅡ were significantly lower than CK when the NO3- concentration was>84 mmol·L-1.The potential activity of PSⅡ was similar to CK when the NO3- concentration was 42 and 126 mmol·L-1,but significantly lower than CK in other treatments.The activities of Mg-ATPase and Ca-ATPase were decreased firstly but increased then when the NO3- concentration was>84 mmol·L-1.

Key words: snow pack, global warming, soil temperature, soil nutrient, alpine forests of west Sichuan