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Free radicals in Carassius auratus liver:Their generation and oxidative stress induced by 2,4,6-trichlorophenol

JI Liang-liang1;LI Fa-yun1;LUO Yi2;MA Xi-ping1;CHEN Zhong-lin1   

  1. 1School of Resources and Environment, Liaoning University, Shenyang 110036, China;
    2College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China
  • Received:2006-03-16 Revised:2006-10-31 Online:2007-01-18 Published:2007-01-18

Abstract: With electron paramagnetic resonance (EPR) technique, this paper studied the free radicals generation and oxidative stress in Carassius auratus liver after 4, 8, 12, 24 and 72 hours of 2,4,6-trichlorophenol (2,4,6-TCP) intraperitoneal injection. The results showed that 2,4,6-TCP significantly promoted the generation of free radicals in C. auratus liver. The superoxide dismutase (SOD) and glutathione-S-transferase (GST) activities were induced significantly, while catalase (CAT) activity was inhibited, and glutathione (GSH) content had little difference with the control. A significant increase was observed in the contents of oxidized glutathione (GSSG) and malondialdehyde (MDA).

Key words: Black soil, Farmyard manure, Urease activity, Dynamic change, Soil nutrient