Abstract: In order to understand the effects of remained enrofloxacin (ENR) in e nvironment on the diversity of soil microbial communities, amplified ribosomal D NA restriction analysis (ARDRA) approach and genomic fingerprinting technique en terobacterial repetitive intergenic consensus sequence-polymerase chain reactio n (ERIC-PCR) were used to analyze the molecular diversity of 16S rDNA from soil bacteria after ENR addition. The results showed that after the ENR addition for 35 days, the total count of soil bacteria was less than that of CK, and decreas ed with increasing ENR concentration. The ARDRA divided the separated soil bacte ria into different operational taxonomic units (OTU) groups, and the count of gr oupⅠ to group Ⅵ was 15, 13, 10, 8, 6, and 6, respectively. Genomic fingerprint ing analysis indicated that the Shannon-Wiener index of group Ⅰ to group Ⅵ wa s 278, 214, 178, 111, 069 and 031, respectively, and the Margalef in dex, Simpson index, and Pielou index of soil microbial community in CK were high er than that in the soils in which ENR was added.

Key words: LANDIS, evergreen broad-leaved forest, natural succession, dominant species, landscape.