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UP-PCR diversity analysis of Fusarium population isolated from greenhouse melon soils.

ZHAO Bai-xia;GAO Zeng-gui;ZHUANG Jing-hua;ZHANG Xiao-fei;ZHAO Hui   

  1. Ministry of Agriculture Key Laboratory of Northern Crop Immunology, College of Plant Protection, Shenyang Agricultural University, Shenyang 110161,China
  • Received:2008-10-10 Revised:1900-01-01 Online:2009-04-20 Published:2009-04-20

Abstract: A total of 112 Fusarium isolates were obtained from 36 soil samples collected from the greenhouse melon fields of Liaoning Province, among which, 11 species were identified by traditional morphological classification and rDNA sequence analysis. Universally Primed PCR (UP-PCR) was conducted to analyze the 25 strains of test Fusarium isolates and 3 strains of positive control Fu sarium isolates. The results indicated that a total of 73 bands appeared after amp lification by using 6 primers, and 66 bands (904%) were polymorphic. The isola tes were clustered into eight groups at the similarity of 0736 by cluster anal ysis, among which, 14 isolates were clustered into one group. It was concluded t hat UP-PCR could present the genetic relationship and difference among Fusari um strains, being able to be used as an assistant method for Fusarium clas sificati on.

Key words:
arthropod,
forest stand type, species-abundance, species-area, restoration area.