Abstract: The thermal tolerance,body temperature,and influence of temperature on locomotor performance of hatchling soft-shelled turtles (Trionyx sinensis) were studied under dry and wet conditions,and the selected body temperature of hatchlings was 28.0 and 30.3 ℃,respectively. Under wet condition,the critical thermal maximum and minimum averaged 40.9 and 7.8 ℃,respectively. In the environments without thermal gradients,the diel variation of body temperature was highly consistent with the variation of both air and water temperatures,and the body temperature was more directly affected by water temperature than by air temperature,which implied that the physiological thermoregulation of hatchling T. sinensis was very weak. In the environments with thermal gradients,hatchling turtles could maintain relatively high and constant body temperatures,primarily through behavioral thermoregulation. The locomotor performance of hatchling turtles was highly dependent on their body temperature. Within a certain range,the locomotor performance increased with increasing body temperature. In our study,the optimal body temperature for locomotor performance was 31.5 ℃,under which,the maximum continuous running distance,running distance per minute,and number of stops per minute averaged 1.87 m, 4.92 m ·min^-1,and 6.2 times·min^-1,respectively. The correspondent values at 33.0 ℃ averaged 1.30 m, 4.28 m ·min^-1,and 7.7 times·min^-1,respectively,which indicated that the locomotor performance of hatchling turtles was impaired at 33.0 ℃. Therefore,extremely high body temperatures might have an adverse effect on locomotor performance of hatchling turtles.