Use of a xylE marker gene to monitor the survival of rhizobia populations in soil

Abstract

Abstract: XylE gene containing plasmid pLV1016 is introduced into Rhizobium fredii QB1130 and Rhizobium leguminosarum bv.viciae B40 by bacterial conjugation,and the expression level of xylE gene in rhizobia is quite high.The use of xyLE gene carried by plasmid pLV1016 to monitor the survival of rhizobia in sterile and non-sterile soils shows that in sterile soil,there is no significant difference(P<0.05) between the survival of strains containing and free of plasmid. Large inoculum quantity is.advantage to the growth and reproduction of bacteria,and the loss rate of plasmid B40(pLV1016):very low.When the inoculation is made with a concentration lower than 10⁶ CFU·g^-1 dry soil,the loss rate of plasmid QB1130(pLV1016) is increased with decreasing inoculation concentration.In non-sterile soil,biotic factors can inhibit the growth of released strains,and large inoculum quantity is advantage to the survival of bacteria. When the inoculation is made with a concentration lower than 10⁶ CFU·g^-1 dry soil, the loss rate of plasmid QB1130(pLV1016)is higher than that of plasmid B40 (PLV1016).

Key words: xylE gene, Monitoring marker, Rhizobia, Survival, photosynthetic physiological characteristics, slope aspect, subalpine meadow, plant leaf trait, microhabitat

Cui Mingxue, Zhang Chenggang, Jin Suying, Zhang Xiaodong, Li Mingqi, Li Ning, Lin Jishang. Use of a xylE marker gene to monitor the survival of rhizobia populations in soil[J]. Chinese Journal of Applied Ecology, 1996, 7(3): 287-292.

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