Abstract: Using polyacrylamide gel electrophoresis (PAGE) technigue, this paper analyzed four isozymes in the seed bank of Glycine soja, and the temporal and spatial dynamics of genetic diversity at isozyme level were studied. Isozymatic analysis, cluster analysis and X²-test revealed that genetic diversity based on the four isozymes existed in the seed bank of G.Soja, and that the diversity mainly originated from environmental difference caused by time. The diversity in the same layer showed a significant difference with time. The seeds scattered in different deep showed different change in their genetic diversity. The variations of isozymes within layers became more and more distinct with time, and the average heterozygosity of seed bank in the same layer appears the same pattern. The results suggest that time takes more significant effect on genetic diversity at isozyme level in the seed bank than space does.

Key words: Glycine soja, Seed bank, Isozyme, Genetic diversity, ecological suitable region, Codonopsis pilosula, maximum entropy model, ecological characteristics