Abstract: This paper presents the effect of some factors as stored time at low temperature, hatching temperature, illumination and hatching medium on the hatching of Brachionus calyciflorus restingeggs. The hatching rate is increased with increasing stored time at low temperautre (5℃), withthe highest rate in 30～50 days. increascd with increasing tempeautre at the range of 10～30℃, and decreased at above 30℃. Illumination is not a necessary factor for hatching. Aftertransferred from old to fresh medium, the resting eggs are stimulated to hatch.

Key words: Brachionus calyciflorus, Resting eggs, Hatching, cumulative effect, ecological effect, interpretative structural modeling (ISM), environmental impact, coal-electricity integration