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Effects of culture condition on fatty acids in Pseudomonas sp

YE Fangting1,YAN Xiaojun1,ZHENG Li2,3,XU Jilin1,CHEN Haimin1    

  1. 1Faculty of Life Science and Biotechnology,Ningbo University,Ningbo 315211, China;2Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;3First Institute Oceanography of SOA,Qingdao 266061,China

  • Received:2004-04-14 Revised:2004-08-02 Online:2005-05-18

Abstract: The investigation on the growth of Pseudomonas sp.and the dynamics of its fatty acids under various culture conditions indicated that the optimum temperature for its growth was 30 ℃,and the best carbon source was starch.13 kinds of fatty acids were detected,among which,c16:1(n7),c15:0, c16:0,c17:0,c18:1(n6),c18:1(n9),9,10cpc17:0 and iso9,10cpc17:0 were the major ones.When cultured at 30 ℃,the relative content of unsaturated fatty acids decreased significantly.Carbon source in the media was the crucial factor affecting the contents of odd- and cyclopropane fatty acids.Cluster analysis revealed that temperature had a more obvious effect than carbon source.

Key words: Beauveria bassiana, Emulsifiable conidia suspensions, Myzus persicae, Field trial, Aphid control