Methods for studying soil microbial diversity

Abstract

Abstract: This paper gave a review on the main methods for studying soil microbial diversity.Traditionally,the analysis of soil microbial communities relied on culturing techniques,using a variety of culture media.However,only a small fraction of the soil microbial community has been cultured and isolated with this approach.Other methods such as Biolog GN analysis,phospholipids fatty acids analysis and nucleic acid-based analysis can be used to study and characterize soil microbes which currently cannot be cultured,and to get more and complete information about soil microbial community.

Key words: Soil, Microbial diversity, Biolog, FLFA, Nucleic acid-based analysis

CLC Number:

S154.3

ZHONG Wenhui, CAI Zucong . Methods for studying soil microbial diversity[J]. Chinese Journal of Applied Ecology, 2004, (5): 899-904.

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