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Chinese Journal of Applied Ecology ›› 2005, Vol. 16 ›› Issue (6): 1099-1103.

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Effects of acetochlor and methamidophos on culturable fungal population and community structure in black soil

LI Xinyu1,2, ZHANG Huiwen1   

  1. 1. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
    2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China
  • Received:2004-12-06 Revised:2005-02-24 Online:2005-06-15 Published:2005-06-15

Abstract: By the methods of CFU and rDNA-PCR-DGGE,this paper studied the dynamic changes of culturable fungal populations,community structure,and population richness in black soil under stress of acetochlor and methamidophos.The results showed that the growth of fungi was promoted under lower concentration acetochlor (50 mg穔g-1),but long-standing inhibited under its higher concentration (250 mg穔g-1).250 mg穔g-1 methamidophos promoted the growth of culturable fungi intensively during 8-week period,resulting in 10 times increase in CFU of fungi than the control,while low concentration methamidophos (50 mg穔g-1) had no evident effect.The richness of culturable fungi in soil treated by medium and high concentration acetochlor was decreased irreversibly.Cluster analysis of fungal rDNA-PCR-DGGE fingerprints profile showed that acetochlor and methamidophos could change the culturable fungal community structure diversely,and the effect of methomidophos was more obvious.

Key words: Acetochlor, Methamidophos, Fungi community, PCR-DGGE, Black soil

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