Introduction of bioluminescence genes into silicate-dissolving bacteria strain NBT

Abstract

Abstract: In this study,silicate-dissolving bacteria NBT strain was grown with 0.25% maltose as carbon source,and the rifampicin-resistance was generated by ultraviolet mutagenesis and streak naturalized to 200 μg·ml^-1.Through triparental cross,the luxAB genes were introduced into NBT-R200 at the help of pRK2013.The luminescence activity of the hybrid strain was tested,which indicated that all colonies had a high luminescence activity and kanamycin-,tetracyctine-and rifampicin-resistance.The NBT-R200 cells prepared from initial logarithmic growth phage were more likely to be sensitive to foreign DNA,and the maximum translocation frequency was up to 6.70×10^-5.In addition,the optimal mating ratio was 1∶1∶1.The potassium release ability from feldspar and the luminescence of luxAB genes marked silicate-dissolving bacteria RL85 strain were stable,and hence,the RL85 was available to rhizosphere microecology researches.

Key words: Silicate-dissolving bacteria, lux marker genes, Triparental cross

CLC Number:

HE Linyan, HUANG Weiyi. Introduction of bioluminescence genes into silicate-dissolving bacteria strain NBT[J]. Chinese Journal of Applied Ecology, 2004, (7): 1241-1244.

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