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SO2-caused oxidative stress and modulation of some signal molecules in wheat

HAO Lin1,ZHANG Huiwen2,XU Xin1,TAO Siyuan1,YU Long1   

  1. 1Department of Biology,Shenyang Normal University,Shenyang 110034, China; 2Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China
  • Received:2004-10-12 Revised:2005-02-28 Online:2005-06-18

Abstract: By adding different concentration SO2 to culture boxes,this paper studied the responses of wheat seedling's superoxide anion radical O2- and antioxidant enzymes to SO2.The results showed that when the concentration of SO2 was 10 and 40 μl·L-1,the O2- content and peroxidase (POD) and catalase(CAT) activities of wheat leaf were increased,while the activity of superoxide dismutase(SOD) was reduced.When the SO2 concentration was 50 μl·L-1, the activities of POD and CAT were decreased,with the top end leaves appeared necrosis,and more fungi multiplied on green leaves.Presoaking wheat seeds with 1 mmol·L-1 salicylic acid (SA) for 6 h or with 10 mmol·L-1 H2O2 for 12 h could efficiently alleviate the oxidative stress caused by SO2,with the O2- content decreased and the activities of test antioxidant enzymes increased.Under SO2 fumigation,ethylene significantly inhibited the activities of test enzymes,and promoted the O2- production rate.When applying SA and ethylene at the same time,SA could almost completely eliminate the influences of ethylene on O2- production and test enzyme activities.

Key words: Riec, Roots, Growth and distribution, Root box, Soil compaction