Abstract: The study on the calatase, polyphenol-oxidase, invertase, urease and phosphatase activities in Paeonia ostii rhizosphere and nonrhizosphere soil of Tongling copper mining showed that all test enzyme activities were higher in rhizosphere than in non-rhizosphere soil. Soil calatase, urease and phosphatase were sensitive to heavy metals pollution, and their activities could be used as the indicators of heavy metals’ joint pollution. The effects of rhizosphere environment on the soil enzyme activities were in the sequence of phosphatase＞urease＞calatase＞invertase＞polyphenoloxidase, and the affecting rate was 131.562%, 92.492%, 87.557%, 59.673% and 34.076%, respectively. The test enzyme activities were negatively correlated with soil heavy metals pollution, and the correlation coefficients were all higher than -0.898, suggesting the inhibitory effects of heavy metals’ joint pollution on soil enzyme activities. P. ostii could effectively improve soil environment, and thus, enhance the activities of soil enzymes.

Key words: Fluoride, Mulberry insects, Mulberry leaves