春季冻融期兴安落叶松林土壤微生物的时间动态及其影响因子

doi:10.13287/j.1001-9332.201908.040

应用生态学报 ›› 2019, Vol. 30 ›› Issue (8): 2757-2766.doi: 10.13287/j.1001-9332.201908.040

春季冻融期兴安落叶松林土壤微生物的时间动态及其影响因子

王楠^1,2, 王传宽^1,2, 邸雪颖¹, 全先奎^1,2*

¹东北林业大学生态研究中心, 哈尔滨 150040;
²东北林业大学森林生态系统可持续经营教育部重点实验室, 哈尔滨 150040

收稿日期:2019-01-19 出版日期:2019-08-15 发布日期:2019-08-15
通讯作者: * E-mail: quanxiankui@nefu.edu.cn
作者简介:王楠,女,1982年生,博士研究生.主要从事森林生态学研究.E-mail:mysky20040216@126.com
基金资助:
中央高校基本科研业务费专项(2572019CP07)和国家“十二五”科技支撑项目(2011BAD37B01)

Temporal dynamics and influencing factors of soil microbes in Larix gmelinii forest soil during spring freezing-thawing period

WANG Nan^1,2, WANG Chuan-kuan^1,2, DI Xue-ying¹, QUAN Xian-kui^1,2*

¹Center for Ecological Research, Northeast Forestry University, Harbin 150040, China;
²Key Laboratory of Sustainable Forest Ecosystem Management, Ministry of Education, Northeast Forestry University, Harbin 150040, China.

Received:2019-01-19 Online:2019-08-15 Published:2019-08-15
Contact: * E-mail: quanxiankui@nefu.edu.cn

摘要/Abstract

摘要： 春季冻融期土壤微生物动态会影响生长季的碳和养分循环.在春季冻融期,每隔3～7 d取样一次,采用磷脂脂肪酸法(PLFA)研究了兴安落叶松林4种土壤基质的微生物群落时间动态.结果表明: 1) 土壤微生物PLFAs总量、各类群的PLFAs量和相对丰度、革兰氏阳性细菌/革兰氏阴性细菌(G⁺/G^–)、饱和脂肪酸/不饱和脂肪酸(S/NS)和细菌/总真菌(真菌+丛枝菌根真菌)(B/F)均存在显著的取样时间差异; 2) 在冻融前期土壤总有机碳(TOC)和土壤全氮(TN)是影响土壤微生物的主要因子,在冻融中期土壤湿度和土壤TOC、TN含量是主要影响因子,在冻融后期土壤微生物受到土壤温湿度、土壤TOC、TN含量及土壤碳氮比(C/N)的共同影响;3) 土壤微生物PLFAs总量、各类群的PLFAs量和相对丰度(细菌丰度除外)、B/F、G⁺/G^-、S/NS在土壤基质间均存在显著差异,土壤TOC、TN和C/N的不同是引起差异的主要环境因素.春季冻融期土壤温湿度和资源有效性是影响土壤微生物群落的主要因子,但影响程度因冻融阶段和微生物类群的不同而存在差异.

Abstract: Soil microbial community dynamics during the spring freezing-thawing period could affect carbon and nutrient cycling in the subsequent growing season. During spring soil freezing-thawing period, we monitored temporal dynamics of soil microbial community in different soil substrates for Larix gmelinii forest using phospholipid fatty acids (PLFAs) as biomarkers every 3-7 days. The results were as followed: 1) the total PLFAs content, the PLFAs content and relative abundance of each soil microbial group, the ratio of Gram-positive bacteria to Gram-negative bacteria (G⁺/G^-), the ratio of saturated PLFAs to unsaturated PLFAs (S/NS) and the ratio of bacteria to total fungi (fungi + arbuscular mycorrhizal fungi) (B/F) all varied among sampling times; 2) soil total organic carbon (TOC) and nitrogen (TN) were the main factors affecting soil microbial community in the early stage of soil freezing-thawing period; soil moisture, TOC and TN were the main driving factors in the middle stage of soil freezing-thawing period; soil microbes were affected by soil tempera-ture, moisture, TOC, TN and C/N in the late stage of soil freezing-thawing period; 3) the total PLFAs content, the PLFAs content and relative abundance of each soil microbial group (except the relative abundance of bacteria), B/F, G⁺/G^- and S/NS all showed significant difference between soil substrates, and soil TOC, TN and C/N were the key determination factors. Soil temperature, moisture, and nutrient availability were the main factors affecting soil microbial community during the spring soil freezing-thawing period, but the degree of influence varied with the freezing-thawing stages and microbial groups.

王楠, 王传宽, 邸雪颖, 全先奎. 春季冻融期兴安落叶松林土壤微生物的时间动态及其影响因子[J]. 应用生态学报, 2019, 30(8): 2757-2766.

WANG Nan, WANG Chuan-kuan, DI Xue-ying, QUAN Xian-kui. Temporal dynamics and influencing factors of soil microbes in Larix gmelinii forest soil during spring freezing-thawing period[J]. Chinese Journal of Applied Ecology, 2019, 30(8): 2757-2766.

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春季冻融期兴安落叶松林土壤微生物的时间动态及其影响因子

Temporal dynamics and influencing factors of soil microbes in Larix gmelinii forest soil during spring freezing-thawing period

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