间作对马铃薯种植土壤硝化作用和硝态氮供应的影响

doi:10.13287/j.1001-9332.202012.023

应用生态学报 ›› 2020, Vol. 31 ›› Issue (12): 4171-4179.doi: 10.13287/j.1001-9332.202012.023

间作对马铃薯种植土壤硝化作用和硝态氮供应的影响

赵薇¹, 伊文博¹, 王顶¹, 吴开贤², 赵平¹, 龙光强^1*, 汤利¹

¹云南农业大学农业农村部云南耕地保育科学观测实验站, 昆明 650201;
²云南农业大学农学与生物技术学院, 昆明 650201

收稿日期:2020-07-14 接受日期:2020-09-20 发布日期:2021-06-15
通讯作者: *E-mail: ynaulong2316@163.com
作者简介:赵薇,女,1996年生,硕士研究生。主要从事施肥与环境效应研究。E-mail:1250340654@qq.com
基金资助:
云南省重点研发计划项目(2018BB015)、国家自然科学基金项目(41967004)、云南省农业联合专项(2017FG001-027)和云南省中青年后备人才项目(2017HB027)资助

Effects of intercropping on soil nitrification and nitrogen supply in potato field.

ZHAO Wei¹, YI Wen-bo¹, WANG Ding¹, WU Kai-xian², ZHAO Ping¹, LONG Guang-qiang^1*, TANG Li¹

¹Yunnan Scientific Observation Station for Cultivated Land Conservation of the Ministry of Agriculture and Rural Affairs, Yunnan Agricultural University, Kunming 650201, China;
²College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China

Received:2020-07-14 Accepted:2020-09-20 Published:2021-06-15
Contact: *E-mail: ynaulong2316@163.com
Supported by:
Key Research and Development Program of Yunnan Province (2018BB015), the National Natural Science Foundation of China (41967004), the Yunnan Agricultural Joint Project (2017FG001-027) and the Yunnan Young and Middle-aged Reserve Talents Project (2017HB027).

摘要/Abstract

摘要： 土壤硝态氮供应对满足作物氮素需求至关重要,但间作如何影响土壤硝态氮供应及其作用机制尚不清楚。本研究基于4个氮水平(N₀, 0 kg·hm^-2; N₁, 62.5 kg·hm^-2; N₂, 125 kg·hm^-2; N₃, 187.5 kg·hm^-2)的马铃薯单作、马铃薯与玉米间作小区试验,分析土壤硝态氮含量与强度、硝化势和氨氧化功能基因丰度的差异,探讨间作影响土壤硝态氮供应和氮调控的机理。结果表明: 土壤硝态氮含量和强度随施氮量增加而升高,但同一施氮水平下间作均低于单作。施氮提高了土壤硝化势,且单作的响应高于间作。土壤中氨氧化细菌(AOB)的amoA基因丰度大于氨氧化古菌(AOA),二者在间作时均随施氮量增加呈现先增加后降低的趋势;相同施氮量下,间作的AOA和AOB基因丰度(除N₂外)均低于单作。相关分析、回归分析和主成分分析显示,马铃薯间作后,土壤AOB、AOA的amoA基因丰度下降,硝化势减弱,导致土壤硝态氮含量和强度降低。因此,间作导致土壤硝态氮供应降低与土壤氮转化的微生物过程有关,间作条件下的马铃薯种植应注意保障土壤氮素供应。

关键词: 间作马铃薯, 氮水平, 硝化势, amoA基因, 硝态氮强度

Abstract: Nitrate supply in soils is essential to meet nitrogen (N) demand of crops. However, how intercropping would affect soil nitrate supply and the underlying mechanisms remain unclear. Based on a field experiment of mono- and inter-cropped potato amended with four N application rates (N₀, 0 kg·hm^-2; N₁, 62.5 kg·hm^-2; N₂, 125 kg·hm^-2; N₃, 187.5 kg·hm^-2), we analyzed the differences in soil nitrate content and intensity, nitrification potential, and ammonia oxidation gene abundance. We further explored the mechanisms underlying the effects of intercropping on nitrate supply and N regulation. The results showed that both content and intensity of soil nitrate increased with N application rate. Under the same N level, nitrate content in intercropping soil was lower than that in monocropping soil. Nitrogen application increased soil nitrification potential, with stronger effects in monocropping than that of intercropping. The amoA gene abundance of AOB in soil was greater than that of AOA. The abundance of amoA gene for both AOA and AOB were increased firstly and then decreased with increasing N application rate when potato intercropped with maize. Under the same N application rate, AOA gene and AOB gene in all treatments (except N₂) in intercropping were lower than those in the monocropping. The amoA gene abundance of soil AOB and AOA decreased and nitrification potential was weakened when potato intercropped with maize, resulting in a decrease of soil nitrate content and intensity. Therefore, intercropping led to a reduction of soil nitrate supply, which was related to the microbial process of soil N transformation. Much attention should be paid to soil N supply under the condition of potato and maize intercropping.

Key words: intercropped potato, N level, nitrification potential, amoA gene, nitrate intensity.

赵薇, 伊文博, 王顶, 吴开贤, 赵平, 龙光强, 汤利. 间作对马铃薯种植土壤硝化作用和硝态氮供应的影响[J]. 应用生态学报, 2020, 31(12): 4171-4179.

ZHAO Wei, YI Wen-bo, WANG Ding, WU Kai-xian, ZHAO Ping, LONG Guang-qiang, TANG Li. Effects of intercropping on soil nitrification and nitrogen supply in potato field.[J]. Chinese Journal of Applied Ecology, 2020, 31(12): 4171-4179.

参考文献

[1] 辛亮. 长期施肥对黄土高原旱地土壤硝化过程及N₂O排放的影响. 硕士论文. 杨凌: 西北农林科技大学, 2012 [Xin L. Effects of Long-term Fertilization on Soil Nitrification and N₂O Emission in the Semiarid Loess Plateau. Master Thesis. Yangling: Northwest A&F University, 2012]
[2] Bartelheimer M, Poschlod P. The response of grassland species to nitrate versus ammonium coincides with their pH optima. Journal of Vegetation Science, 2014, 25: 760-770
[3] 贺纪正, 张丽梅. 土壤氮素转化的关键微生物过程及机制. 微生物学通报, 2013, 40(1): 98-108 [He J-Z, Zhang L-M. Key processes and microbial mechanisms of soil nitrogen transformation. Microbiology China, 2013, 40(1): 98-108]
[4] 郭佳. 典型环境因子对硝化作用和氨氧化微生物生理生态的影响. 硕士论文. 重庆: 西南大学, 2016 [Guo J. The Influence of Typical Environmental Factors on Nitrification and Physiological Ecology of Ammonia-oxidizers. Master Thesis. Chongqing: Southwest University, 2016]
[5] Philippot L, Hallin S. Towards food, feed and energy crops mitigating climate change. Trends in Plant Science, 2011, 16: 476-480
[6] Könneke M, Schubert DM, Brown PC, et al. Ammonia-oxidizing archaea use the most energy-efficient aerobic pathway for CO₂ fixation. Proceedings of the National Academy of Sciences of the United States of America, 2014, 111: 8239-8244
[7] Zhou L, Wang S, Zou Y, et al. Species, abundance and function of ammonia-oxidizing archaea in inland waters across China. Scientific Reports, 2015, 5: 15969
[8] Prosser JI. Autotrophic nitrification in bacteria. Advances in Microbial Physiology, 1990, 30: 125-181
[9] Ai C, Liang GQ, Sun JW, et al. Different roles of rhizosphere effect and long-term fertilization in the activity and community structure of ammonia oxidizers in a calcareous fluvo-aquic soil. Soil Biology and Biochemistry, 2013, 57: 30-42
[10] 李秋红, 吴凤芝, 景芳毅. 套作对黄瓜根际土壤细菌群落结构的多样性影响. 土壤通报, 2010, 41(1): 47-52 [Li Q-H, Wu F-Z, Jing F-Y. T-RFLP analysis of effects of intercropping on cucumber rhizosphere soil bacterial community structure diversity. Chinese Journal of Soil Science, 2010, 41(1): 47-52]
[11] Fan F, Zhang F, Lu Y. Linking plant identity and interspecific competition to soil nitrogen cycling through ammonia oxidizer communities. Soil Biology and Biochemistry, 2011, 43: 46-54
[12] 雍太文, 苏本营, 刘文钰, 等. 减量施氮对玉米-大豆套作系统中作物产量的影响. 作物学报, 2014, 40(9): 1629-1638 [Yong T-W, Su B-Y, Liu W-Y, et al. Effect of reduced N application on crop yield in maize-soybean intercropping system. Acta Agronomica Sinica, 2014, 40(9): 1629-1638]
[13] Zhao M, Jones CM, Meijer J, et al. Intercropping affects genetic potential for inorganic nitrogen cycling by root-associated microorganisms in Medicago sativa and Dactylis glomerata. Applied Soil Ecology, 2017, 119: 260-266
[14] 雍太文. “麦/玉/豆”套作体系的氮素吸收利用特性及根际微生态效应研究. 硕士论文. 雅安: 四川农业大学, 2009 [Yong T-W. Analysis of the Nitrogen Uptake and Utilization, Rhizosphere Micro-Ecology in the “Wheat/Maize/Soybean” Relay-Cropping System. Master Thesis. Ya'an: Sichuan Agricultural University, 2009]
[15] 王玉琪, 张建军, 朱国辉, 等. 不同形态氮素培养下水稻叶片中蛋白质差异表达. 植物生理与分子生物学学报, 2006, 32(4): 403-410 [Wang Y-Q, Zhang J-J, Zhu G-H, et al. Differential expression of proteins in rice leaves cultivated with different forms of nitrogen nutrients. Journal of Plant Physiology and Molecular Biology, 2006, 32(4): 403-410]
[16] 郑成岩, 于振文, 王东, 等. 耕作方式对冬小麦氮素积累与转运及土壤硝态氮含量的影响. 植物营养与肥料学报, 2012, 18(6): 1303-1311 [Zheng C-Y, Yu Z-W, Wang D, et al. Effects of tillage practices on nitrogen accumulation and translocation in winter wheat and NO₃^--N content in soil. Plant Nutrition and Fertilizer Science, 2012, 18(6): 1303-1311]
[17] 吴开贤, 安瞳昕, 范志伟, 等. 土壤氮异质性与种间地上竞争对玉米和马铃薯生长的影响. 中国生态农业学报, 2012, 20(12): 1571-1578 [Wu K-X, An T-X, Fan Z-W, et al. Maize and potato growth responses to heterogeneous nitrogen and shoot competition. Chinese Journal of Eco-Agriculture, 2012, 20(12): 1571-1578]
[18] 鲍士旦. 土壤农化分析. 北京: 中国农业出版社, 2000: 42-108 [Bao S-D. Soil and Agrochemistry Analy-sis. Beijing: China Agriculture Press, 2000: 42-108]
[19] Chu H, Fujii T, Morimoto S, et al. Community structure of ammonia-oxidizing bacteria under long-term application of mineral fertilizer and organic manure in a sandy loam soil. Applied and Environmental Microbiology, 2007, 73: 485-491
[20] He JZ, Shen JP, Zhang LM, et al. Quantitative analyses of the abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea of a Chinese upland red soil under long-term fertilization practices. Environmental Microbiology, 2007, 9: 2364-2374
[21] 马兴华, 于振文, 梁晓芳, 等. 施氮量和底施追施比例对土壤硝态氮和铵态氮含量时空变化的影响. 应用生态学报, 2006, 17(4): 630-634 [Ma X-H, Yu Z-W, Liang X-F, et al. Effects of nitrogen application rate and its basal-/top-dressing ratio on spatio-temporal variations of soil NO₃^--N and NH₄ ⁺-N contents. Chinese Journal of Applied Ecology, 2006, 17(4): 630-634]
[22] 秦宇坤, 李鹏程, 郑苍松, 等. 氮肥施用量对棉花产量及土壤脲酶活性的影响. 新疆农业大学学报, 2018, 41(2): 86-92 [Qin Y-K, Li P-C, Zheng C-S, et al. Effects of nitrogen application rate on the cotton yield and soil urease activity. Journal of Xinjiang Agricultural University, 2018, 41(2): 86-92]
[23] Dandie CE, Burton DL, Zebarth BJ, et al. Changes in bacterial denitrifier community abundance over time in an agricultural field and their relationship with denitrification activity. Applied and Environmental Microbiology, 2008, 74: 5997-6005
[24] 何杰, 李冰, 王昌全, 等. 不同施氮处理对水稻油菜轮作土壤氮素供应与作物产量的影响. 中国农业科学, 2017, 50(15): 2957-2968 [He J, Li B, Wang C-Q, et al. Effects of different nitrogen fertilization treatments on soil nitrogen supply and yield in rice-rape rotation. Scientia Agricultura Sinica, 2017, 50(15): 2957-2968]
[25] 叶优良, 李隆, 索东让. 小麦/玉米和蚕豆/玉米间作对土壤硝态氮累积和氮素利用效率的影响. 生态环境, 2008, 17(1): 377-383 [Ye Y-L, Li L, Suo D-R. Effect of wheat/maize and faba bean/maize intercropping on soil nitrate nitrogen concentration and accumulation. Ecology and Environment, 2008, 17(1): 377-383]
[26] 赵胜利, 龙光强, 杨超, 等. 施氮对玉米//马铃薯间作作物氮累积和分配的影响. 云南农业大学学报: 自然科学版, 2016, 31(5): 886-894 [Zhao S-L, Long G-Q, Yang C, et al. Effects of nitrogen application on nitrogen accumulation and distribution of crops in maize and potato intercropping. Journal of Yunnan Agricultural University: Natural Science, 2016, 31(5): 886-894]
[27] 吴开贤, 安瞳昕, 范志伟, 等. 玉米马铃薯的间作优势和种间关系对氮投入的响应. 植物营养与肥料学报, 2012, 18(4): 1006-1012 [Wu K-X, An T-X, Fan Z-W, et al. Effects of nitrogen input on yields advantage and interaction of the maize and potato intercropping. Plant Nutrition and Fertilizer Science, 2012, 18(4): 1006-1012]
[28] 路璐, 何燕. 不同林分土壤中氨氧化微生物的群落结构和硝化潜势差异及其驱动因子. 南方农业学报, 2018, 49(11): 2169-2176 [Lu L, He Y. The diffe-rence of ammonia-oxidizing microorganism communities structure and nitrification potential in soils of different forest stands and their driving factors. Journal of Sou-thern Agriculture, 2018, 49(11): 2169-2176]
[29] 吕玉, 周龙, 龙光强, 等. 不同氮水平下间作对玉米土壤硝化势和氨氧化微生物数量的影响. 环境科学, 2016, 37(8): 3229-3236 [Lyu Y, Zhou L, Long G-Q, et al. Effect of different nitrogen rates on the nitrification potential and abundance of ammonia-oxidizer in intercropping maize soils. Environmental Science, 2016, 37(8): 3229-3236]
[30] Martenshabbena W, Berube PM, Urakawa H, et al. Ammonia oxidation kinetics determine niche separation of nitrifying archaea and bacteria. Nature, 2009, 461: 976-979
[31] Carey CJ, Dove NC, Beman JM, et al. Meta-analysis reveals ammonia-oxidizing bacteria respond more strongly to nitrogen addition than ammonia-oxidizing archaea. Soil Biology and Biochemistry, 2016, 99: 158-166
[32] Song YN, Zhang FS, Marschner P, et al. Effect of intercropping on crop yield and chemical and microbiological properties in rhizosphere of wheat (Triticum aestivum L.), maize (Zea mays L.), and faba bean (Vicia faba L.). Biology and Fertility of Soils, 2007, 43: 565-574

间作对马铃薯种植土壤硝化作用和硝态氮供应的影响

Effects of intercropping on soil nitrification and nitrogen supply in potato field.

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	李红强, 姚荣江, 杨劲松, 王相平, 谢文萍, 张新. 滨海盐渍农田土壤硝化势特征及其影响因素 [J]. 应用生态学报, 2022, 33(8): 2205-2212.
[2]	何雪菲, 黄战, 张文太, 陈波浪, 周妍慧子, 柴仲平. 施氮水平对库尔勒香梨光合产物分配的影响 [J]. 应用生态学报, 2020, 31(8): 2637-2643.
[3]	吴讷, 邵嘉薇, 盛荣, 汤亚芳, 张文钊, 魏文学. 水稻分蘖期和孕穗期根际反硝化菌群落结构及功能变化 [J]. 应用生态学报, 2019, 30(4): 1344-1350.
[4]	张倩, 牛文全, 杜娅丹, 崔冰晶. 加气灌溉对不同施氮水平的设施甜瓜土壤CO₂和N₂O排放的影响 [J]. 应用生态学报, 2019, 30(4): 1319-1326.
[5]	马连坤, 董坤, 朱锦惠, 董艳. 小麦与蚕豆间作系统施氮对蚕豆赤斑病发生和冠层微气候的影响 [J]. 应用生态学报, 2019, 30(3): 951-960.
[6]	王芬, 田歌, 刘晶晶, 葛顺峰, 姜远茂. 富士苹果萌芽至新梢旺长期肥料氮去向和土壤氮库盈亏 [J]. 应用生态学报, 2018, 29(3): 931-937.
[7]	彭玲, 刘晶晶, 王芬, 葛顺峰, 姜远茂. 硝酸盐供应水平对平邑甜茶幼苗生长、光合特性与¹⁵N吸收、利用的影响 [J]. 应用生态学报, 2018, 29(2): 522-530.
[8]	陈倩, 丁宁, 朱占玲, 彭玲, 葛顺峰, 姜远茂. 供氮水平对不同砧穗组合苹果叶片衰老及¹³C、¹⁵N分配利用的影响 [J]. 应用生态学报, 2017, 28(7): 2239-2246.
[9]	陈倩, 丁宁, 彭玲, 葛顺峰, 姜远茂. 供氮水平对矮化苹果¹⁵N-尿素吸收、利用、损失及产量和品质的影响 [J]. 应用生态学报, 2017, 28(7): 2247-2253.
[10]	朱锦惠, 董艳, 肖靖秀, 郑毅, 汤利. 小麦与蚕豆间作系统氮肥调控对小麦白粉病发生及氮素累积分配的影响 [J]. 应用生态学报, 2017, 28(12): 3985-3993.
[11]	唐偲頔,张政,蔡小真,郭剑芬,杨玉盛. 增温和隔离降雨对亚热带森林土壤N₂O通量的影响 [J]. 应用生态学报, 2017, 28(10): 3119-3126.
[12]	张自常, 谷涛, 李永丰, 杨霞. 不同氮水平下不同种稗草对水稻产量形成的影响 [J]. 应用生态学报, 2016, 27(11): 3559-3568.
[13]	余意,杨其长,刘文科**. LED短期连续光照与氮营养对水培生菜品质的影响 [J]. 应用生态学报, 2015, 26(11): 3361-3366.
[14]	董艳1,杨智仙1,董坤2,汤利1,郑毅1,3**,胡国斌1. 施氮水平对蚕豆枯萎病和根际微生物代谢功能多样性的影响 [J]. 应用生态学报, 2013, 24(4): 1101-1108.
[15]	郑成岩,于振文,张永丽,王东,许振柱. 不同施氮水平下灌水量对小麦水分利用特征和产量的影响 [J]. 应用生态学报, 2010, 21(11): 2799-2805.