松毛虫虫食叶和排泄物对土壤激发效应的影响

doi:10.13287/j.1001-9332.202303.012

应用生态学报 ›› 2023, Vol. 34 ›› Issue (3): 770-776.doi: 10.13287/j.1001-9332.202303.012

松毛虫虫食叶和排泄物对土壤激发效应的影响

黄雪梅¹, 陈龙池^2,5, 田宁¹, 关欣^2,5, 胡亚林³, 黄苛^2,5, 宿秀江⁴, 陶晓^1*

¹安徽农业大学林学与园林学院, 合肥 230036;
²中国科学院沈阳应用生态研究所, 中国科学院森林生态与管理重点实验室, 中国科学院会同森林生态实验站, 沈阳 110016;
³福建农林大学林学院, 森林生态稳定同位素研究中心, 福州 350002;
⁴湖南白云山国家级自然保护区管理局, 湖南保靖 416500;
⁵亚热带森林生态系统结构与服务功能湖南省重点实验室, 湖南会同 418300

收稿日期:2022-06-28 接受日期:2023-01-05 发布日期:2023-09-15
通讯作者: *E-mail: hyytaoxiao@163.com
作者简介:黄雪梅, 女, 1998年生, 硕士研究生。主要从事土壤激发效应研究。E-mail: 18325848260@163.com
基金资助:
国家重点研发计划项目(2021YFD2201303)和国家自然科学基金项目(42077094)

Effect of leaves damaged by Dendrolimus punctatus and insect frass on soil priming effect.

HUANG Xuemei¹, CHEN Longchi^2,5, TIAN Ning¹, GUAN Xin^2,5, HU Yalin³, HUANG Ke^2,5, SU Xiujiang⁴, TAO Xiao^1*

¹College of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230036, China;
²Huitong Experimental Station of Forest Ecology, Key Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
³Forest Ecology and Stable Isotope Research Center, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
⁴Hunan Baiyunshan National Nature Reserve Administration, Baojing 416500, Hunan, China;
⁵Hunan Key Laboratory for Structure and Ecosystem Service of Subtropical Forest, Huitong 418300, Hunan, China

Received:2022-06-28 Accepted:2023-01-05 Published:2023-09-15

摘要/Abstract

摘要： 昆虫冠层取食导致大量的虫食叶和排泄物输入到地表,对森林土壤碳循环产生重要影响。然而,目前关于虫食叶和排泄物影响土壤激发效应的研究仍十分匮乏。本研究以马尾松为对象,应用¹³C同位素自然丰度法研究马尾松凋落叶、松毛虫虫食叶和排泄物添加对土壤激发效应强度和方向的影响。结果表明:马尾松凋落叶、虫食叶和排泄物添加均促进土壤有机碳的分解,即产生正激发效应,且各处理之间差异显著。松毛虫排泄物诱导的累积激发效应最大,虫食叶次之,马尾松凋落叶诱导的累积激发效应最小。线性回归分析表明,在培养前期,激发强度与添加物全磷、缩合单宁、缩合单宁/P以及总酚含量呈显著正相关,与C/N、木质素/N、C/P、木质素/P呈显著负相关;在培养后期,激发强度与木质素含量呈显著负相关。综上,松毛虫虫害产生的虫食叶和排泄物提高了土壤激发效应强度,且在不同培养阶段控制激发强度的主导因子不同,这有助于加深对虫害影响森林土壤碳循环的认知,并提高对虫害影响森林生态系统碳汇的估算精度。

关键词: ¹³C自然丰度, 有机碳分解, 激发效应, 马尾松, 排泄物

Abstract: Insect herbivory in the forest canopy leads to a large amount of damaged leaves and frass input to soil, with consequence on soil carbon cycle. However, the influence of damaged leaves and frass from insect canopy herbivory on the soil priming effect is unclear. We examined the effects of leaf litter, leaf damage caused by Dendrolimus punctatus, and insect frass on soil priming effect by using the ¹³C natural abundance technique. The results showed that the addition of leaf litter, damaged leaves, and frass significantly increased native soil organic carbon mineralization, producing a positive priming effect. Moreover, significant differences were observed among treatments. The accumulative priming effect induced by frass was the largest, followed by damaged leaves, and that of leaf litter was the smallest. The priming effect was positively correlated with total P, condensed tannin, total phenolic content, and the ratio of condensed tannin to P (condensed tannin/P), and negatively correlated with C/N, lignin/N, C/P, and lignin/P in the early stage of incubation. There was a significant negative correlation between the priming effect and lignin content in the later stage of incubation. Our results indicated that damaged leaves and frass increased the magnitude of positive priming effect, which was influenced by different factors at different incubation stages. Our results would strengthen the understanding in the effects of insect herbivory on soil carbon cycling in forests, and improve the accuracy of the assessment of its effects on forest carbon sink.

Key words: ¹³C natural abundance, organic carbon decomposition, priming effect, Pinus massoniana, insect frass

黄雪梅, 陈龙池, 田宁, 关欣, 胡亚林, 黄苛, 宿秀江, 陶晓. 松毛虫虫食叶和排泄物对土壤激发效应的影响[J]. 应用生态学报, 2023, 34(3): 770-776.

HUANG Xuemei, CHEN Longchi, TIAN Ning, GUAN Xin, HU Yalin, HUANG Ke, SU Xiujiang, TAO Xiao. Effect of leaves damaged by Dendrolimus punctatus and insect frass on soil priming effect.[J]. Chinese Journal of Applied Ecology, 2023, 34(3): 770-776.

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松毛虫虫食叶和排泄物对土壤激发效应的影响

Effect of leaves damaged by Dendrolimus punctatus and insect frass on soil priming effect.

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