xylE基因用于监测根瘤菌在土壤中存活的研究

应用生态学报 ›› 1996, Vol. 7 ›› Issue (3): 287-292.

xylE基因用于监测根瘤菌在土壤中存活的研究

崔明学, 张成刚, 靳素英, 张晓东, 李明祺, 李宁, 蔺继尚

中国科学院沈阳应用生态研究所, 沈阳110015

收稿日期:1995-12-19 修回日期:1996-02-02 出版日期:1996-07-25 发布日期:1996-07-25
基金资助:
国家自然科学基金资助项目

Use of a xylE marker gene to monitor the survival of rhizobia populations in soil

Cui Mingxue, Zhang Chenggang, Jin Suying, Zhang Xiaodong, Li Mingqi, Li Ning, Lin Jishang

Institute of Applied Ecology, Academia Sinica, Shenyang 110015

Received:1995-12-19 Revised:1996-02-02 Online:1996-07-25 Published:1996-07-25

摘要/Abstract

摘要： 通过细菌接合将质粒pLV1016(含xylE基因)转移至Rhizobium fredii QB1130和Rhizobium leguminosarum bv.viciae B40,xylE基因在根瘤菌内表达活性较高.质粒pLV1016携带的XylE基因用于监测根瘤菌在灭菌和未灭菌土壤中的存活,结果表明,在灭菌土壤中含质粒与不含质粒菌株存活菌数量之间无显着差异(P<0.05),大接种量有利于细菌的生长与繁殖,B40(pLV1016)质粒丢失比例很低.以低于10⁶CFU·g^-1干土浓度接种时,QB1130(pLV1016)质粒丢失率随接种浓度的降低而增大.未灭菌土壤中生物因素抑制了释放菌株的生长,大接种量有利于细菌存活.以低浓度(10⁶CFU·g^-1干土)接种时,QB1130(pLV1016)质粒丢失比例高于B40(pLV1016).

关键词: xylE基因, 监测标记, 根瘤菌, 存活, 光合生理特征, 植物叶性状, 坡向, 微生境, 亚高寒草甸

Abstract: XylE gene containing plasmid pLV1016 is introduced into Rhizobium fredii QB1130 and Rhizobium leguminosarum bv.viciae B40 by bacterial conjugation,and the expression level of xylE gene in rhizobia is quite high.The use of xyLE gene carried by plasmid pLV1016 to monitor the survival of rhizobia in sterile and non-sterile soils shows that in sterile soil,there is no significant difference(P<0.05) between the survival of strains containing and free of plasmid. Large inoculum quantity is.advantage to the growth and reproduction of bacteria,and the loss rate of plasmid B40(pLV1016):very low.When the inoculation is made with a concentration lower than 10⁶ CFU·g^-1 dry soil,the loss rate of plasmid QB1130(pLV1016) is increased with decreasing inoculation concentration.In non-sterile soil,biotic factors can inhibit the growth of released strains,and large inoculum quantity is advantage to the survival of bacteria. When the inoculation is made with a concentration lower than 10⁶ CFU·g^-1 dry soil, the loss rate of plasmid QB1130(pLV1016)is higher than that of plasmid B40 (PLV1016).

Key words: xylE gene, Monitoring marker, Rhizobia, Survival, photosynthetic physiological characteristics, slope aspect, subalpine meadow, plant leaf trait, microhabitat

崔明学, 张成刚, 靳素英, 张晓东, 李明祺, 李宁, 蔺继尚. xylE基因用于监测根瘤菌在土壤中存活的研究[J]. 应用生态学报, 1996, 7(3): 287-292.

Cui Mingxue, Zhang Chenggang, Jin Suying, Zhang Xiaodong, Li Mingqi, Li Ning, Lin Jishang. Use of a xylE marker gene to monitor the survival of rhizobia populations in soil[J]. Chinese Journal of Applied Ecology, 1996, 7(3): 287-292.

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