无花果曲霉原生质体形成与再生条件的探讨

应用生态学报 ›› 1998, Vol. 9 ›› Issue (4): 440-442.

无花果曲霉原生质体形成与再生条件的探讨

宛晓春¹, 李平¹, 丁霄霖², 陶文沂²

1. 安徽农业大学农业部茶叶生物技术重点开放实验室, 合肥230036;
2. 无锡轻工大学, 无锡214036

收稿日期:1998-02-09 修回日期:1998-05-11 出版日期:1998-07-25 发布日期:1998-07-25
基金资助:
国家“八五”科技攻关资助项目(85-609-03-02)

Optimized conditions for protoplast formation and regeneration of Aspergillus ficuum

Wan Xiaochun¹, Li Ping¹, Ding Xiaolin², Tao Wenyi²

1. Key Laboratory of Tea Biotechnology of Ministry of Agriculture, Anhui Agricultural University, Hefei 230036;
2. Wuxi Light Industry University, Wuxi 214036

Received:1998-02-09 Revised:1998-05-11 Online:1998-07-25 Published:1998-07-25

摘要/Abstract

摘要： 根据正交试验得出无花果曲霉原生质体形成的最佳条件,用1%的混合酶液(0.5%纤维素酶+0.25%蜗牛酶+0. 25%溶菌酶)作用无花果曲霉菌体细胞,原生质体产量达3.2×10⁷个·ml^-1, 渗透压稳定剂为0.6mol·L^-1KCl于0.2mol·L^-1PO₄³⁺(pH5.8)中,酶解时间和酶解温度分别为3.0h、30℃。比较不同酶解时间、再生稳定剂和碳源等因素对原生质体再生的影响,可确定最佳再生条件,再生率达30%以上。

关键词: 无花果曲霉, 原生质体形成与再生

Abstract: Orthogonal experiments were designed to study the optimized conditions for protoplast formation of Aspergillus ficuum .A 1% mixed solution of enzymes (0.5% cellulase + 0.25% snail enzyme + 0.25% lysozyme) was used to treat the body cells of A.ficuum, and the yield of protoplast was up to 3.2 × 10⁷ individuals·ml^-1 .The optimum osmotic stabilizer was 0.6 mol·L^-1 KCl containing 0.2 mol·L^-1 PO₄³⁺ (pH5.8), and the optimum duration and optimum temperature for enzymatic action was 3.0h and 30℃, respectively.The optimal conditions for protoplast regeneration were also obtatined, and the regeneration rate was over 30%.

Key words: Aspergillus ficuum, Protoplast formation and regeneration

宛晓春, 李平, 丁霄霖, 陶文沂. 无花果曲霉原生质体形成与再生条件的探讨[J]. 应用生态学报, 1998, 9(4): 440-442.

Wan Xiaochun, Li Ping, Ding Xiaolin, Tao Wenyi. Optimized conditions for protoplast formation and regeneration of Aspergillus ficuum[J]. Chinese Journal of Applied Ecology, 1998, 9(4): 440-442.