关键词: P、Zn, 交互作用, 小麦悬浮细胞

Abstract: This paper studied the accumulation,distribution and interaction of phosphorus and zinc in the asexual cells of wheat cultured in MS solution with three levels of phosphorus(0.5,1.5,3.5 mmol·L^-1) and two levels of zinc(0,60 μmol·L^-1).An application of 60μmol·L^-1 Zn and 0.5～1.5mmol·L^-1 P increased the reproduction and the dry matter yield of wheat cells,while the supply with no Zn or high P(3.5 mmol·L^-1) showed an inhibited effect.The accumulation of P and Zn in cells increased with increasing P and Zn supply. In comparison with Zn-deficiency,an application of 60μmol·L^-1 Zn reduced the P content in cells cultured for 7 days. 0.5～1.5 mmol·L^-1 P enhanced Zn accumulation in cells, but decreased it under 3.5 mmol·L^-1 P. As much as 83.9%～88.3% of the total P was associated with thecell walls, and the more the P supply,the greater the P content in them.The results also showed more than half of Zn (52.0～60.5％)in cells was examined in cell walls.In contrast to the cell walls,the contents of P and Zn in vacuoles were much lower,with 2.3～3.8% and 10.6～30% respectively.

Key words: Phosphorus, Zinc, Interaction, Wheat suspention cell