镧对烟草愈伤组织和油菜幼根钙含量的影响

应用生态学报 ›› 1999, Vol. 10 ›› Issue (6): 739-742.

镧对烟草愈伤组织和油菜幼根钙含量的影响

张家荣, 顾月华, 罗建平, 赵贵文

中国科学技术大学生命科学学院, 合肥230026

收稿日期:1999-03-08 修回日期:1999-06-03 出版日期:1999-11-25 发布日期:1999-11-25
通讯作者: 顾月华
作者简介:张家荣,女,35岁,博士研究生,主要从事植物生理及细胞分子生物学研究,发表论文5篇.E-mail:ymtea@hf.ah.cn
基金资助:
安微省科委“九五”重点项目

Effect of La on Ca content in tobacco callus and oilseed rape seedling root

Zhang Jiarong, Gu Yuehua, Luo Jianping, Zhao Guiwen

University of Science and Technology of China, Hefei 230026

Received:1999-03-08 Revised:1999-06-03 Online:1999-11-25 Published:1999-11-25

摘要/Abstract

摘要： MS培养基中添加镧(La)(0.01～0.10mmol·L^-1)培养烟草愈伤组织,其总Ca²⁺及原生质体Ca²⁺含量明显比对照(不加La)低;长时间(继代培养30d)较短时间培养(悬浮培养24h)低。低浓度(0.01～0.051mol·L^-1)La³⁺使细胞壁中Ca²⁺含量高于对照并随浓度提高而增加,高浓度(0.101mol·L^-1)则减少。与Ce²⁺相比较,La³⁺的排Ca²⁺作用稍弱于Ce³⁺.以不同浓度La(NO₃)₃溶液浸种,油菜幼根总Ca²⁺及原生质体中Ca²⁺含量变化与上述类似。La³⁺使烟草愈伤组织褐化明显,生长量比对照低。

关键词: La³⁺, Ce³⁺, Ca²⁺, 烟草, 油菜

Abstract: The Ca contents in tobacco callus and in its protoplasts were lower in La contained (0.01～0.10mmol·L^-1) MSmedium than in the control (without La addition). As the culture time increased from 1 day to 30 days, the Ca contents declined rapidly. At low La concentration (0.01～0.05mmol·L^-1), the Ca content in cell wall was higher than that of the control, and increased with increasing La concentration; while at high La concentration (0.10mmol·L^-1), it decreased. Compared with Ce, La had a weaker effect on Ca exudation. After oilseed rape seeds were soaked with different concentrations of La(NO₃)₃ solution, the dynamics of Ca content in their seedling roots and protoplasts were the same as above mentioned. La made the tobacco callus quickly browned, and the increment of fresh weight was lower than that of the control.

Key words: La³⁺, Ce³⁺, Ca²⁺, Tobacco, Oilseed rape

张家荣, 顾月华, 罗建平, 赵贵文. 镧对烟草愈伤组织和油菜幼根钙含量的影响[J]. 应用生态学报, 1999, 10(6): 739-742.

Zhang Jiarong, Gu Yuehua, Luo Jianping, Zhao Guiwen . Effect of La on Ca content in tobacco callus and oilseed rape seedling root[J]. Chinese Journal of Applied Ecology, 1999, 10(6): 739-742.

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