海洋细菌抗菌和细胞毒活性的初步研究

摘要/Abstract

摘要： 从不同海域的生物、海水和海泥中分离海洋细菌,利用琼脂扩散法和MTT法对细菌培养液的乙酸乙酯提取物进行了抗菌和细胞毒活性筛选,比较了活性菌株与来源的相关性.结果表明,在分离的341株海洋细菌中,42株细菌的代谢产物具有抗菌活性,7株具有细胞毒活性,其中来源于海洋无脊椎动物和海藻的活性菌株比例(22%和11%)大于来源于海水和海泥的细菌(7%和5%).细菌分类鉴定结果显示,具有活性的细菌大部分属于假单胞菌属、发光杆菌属、梭状芽孢杆菌属、交替单胞菌属和黄杆菌属.

关键词: 海洋细菌, 抗菌, 细胞毒

Abstract: Marine bacteria were isolated from marine organisms including invertebrates and seaweeds and from sea water and sediments of different sea area.Ethyl acetate extractions of marine bacterial fermentation were screened for antimicrobial and cytotoxicity activities by using the methods of agar diffusion and MTT assay,and the relationship of active bacteria and their origin was studied.The results showed that 42 strains among total 341 isolated marine bacteria had antimicrobial activity,and 7 strains had cytotoxicity activity.The proportion of active bacteria associated with marine invertebrates and seaweeds (22% and 11%) was higher than that isolated from sea water and sediments (7% and 5%).The most active marine bacteria were assigned to the Pseudomonas,Photobacterium,Clostridium,Alteromonas and Flavobacterium group.

Key words: Marine bacteria, Antibacterial, Cytotoxicity

中图分类号:

Q938.8

郑立, 林伟, 严小军, 陈海敏. 海洋细菌抗菌和细胞毒活性的初步研究[J]. 应用生态学报, 2004, (9): 1633-1636.

ZHENG Li, LIN Wei, YAN Xiaojun, CHEN Haimin . A primary study on antimicrobial and cytotoxic activity of marine bacteria[J]. Chinese Journal of Applied Ecology, 2004, (9): 1633-1636.

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