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应用生态学报 ›› 2012, Vol. 23 ›› Issue (02): 511-518.

• 研究论文 • 上一篇    下一篇

几种经济作物根际拮抗细菌的多样性

徐莹莹1,杜秉海1,2,姚良同1,靳奉理1,王翠翠1,王〓璇1,丁延芹1**   

  1. 1山东农业大学生命科学学院微生物学系, 山东泰安 271018; 2山东省农业微生物重点实验室, 山东泰安 271018
  • 出版日期:2012-02-18 发布日期:2012-02-18

Diversity of antagonistic bacteria isolated from rhizosphere of several cash crops.

XU Ying-ying1, DU Bing-hai1,2, YAO Liang-tong1, JIN Feng-li1, WANG Cui-cui1, WANG Xuan1, DING Yan-qin1   

  1. 1Department of Microbiology, College of Life Science, Shandong Agricultural University, Tai’an 271018, Shandong, China; 2 Shandong Province Key Laboratory for Agriculture Microbiology,  Tai’an 271018, Shandong, China
  • Online:2012-02-18 Published:2012-02-18

摘要: 采用抗菌谱测定以及BOXAIR-PCR、生理生化特征和16S rDNA序列分析等方法对分离自10种作物根际的55株拮抗细菌的多样性及主要拮抗菌类群进行了分析.结果表明: 根际拮抗细菌的拮抗作用具有丰富的多样性;BOXAIR-PCR分析中供试菌在721%相似性水平上可以聚为7个群,850%相似性水平上聚为25个群;所有供试根际拮抗菌分别属于芽孢杆菌属(Bacillus)芽孢杆菌属(Paenibacillus)、短芽孢杆菌属(Brevibacillus)、假单胞菌属(Pseudomonas)和产碱菌属(Alcaligenes).作物根际拮抗菌具有丰富的遗传多样性和拮抗性能多样性.

关键词: 根际, 抗菌谱, BOXAIR-PCR, 16S rDNA, 多样性

Abstract: By adopting antimicrobial spectrum test, BOXAIR-PCR, physiological and biochemical, and 16S rDNA sequencing analysis, this paper analyzed the diversity of 55 antagonistic bacterial strains isolated from the  rhizosphere  of 10 cash crops. There was a high diversity of the antagonism of the strains. Based on BOXAIR-PCR, all the strains were clustered into 7 groups at the similarity level of 721%, and divided into 25 groups at the similarity level of 850%. All the strains belonged to Bacillus, Paenibacillus, Brevibacillus, Pseudomonas and Alcaligenes, respectively. The antagonistic bacteria isolated from the rhizosphere had high genetic diversity and high diversity in antagonistic activity.

Key words: rhizosphere, antimicrobial spectrum, BOXAIR-PCR, 16S rDNA, diversity