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诱导处理小麦对蚜虫生长发育的影响及小麦特异基因的表达

祝传书;赵惠燕   

  1. 西北农林科技大学植物保护学院,杨凌 712100
  • 收稿日期:2005-04-30 修回日期:2005-08-30 出版日期:2006-04-18 发布日期:2006-04-18

Effects of elicitors on aphid growth and development and on specific genes expression in wheat

ZHU Chuanshu;ZHAO Huiyan   

  1. College of Plant Protection,Northwest Agriculture and Forestry University,Yangling 712100,China

  • Received:2005-04-30 Revised:2005-08-30 Online:2006-04-18 Published:2006-04-18

摘要: 分析了麦二叉蚜在小麦98-10-30上不同诱导处理后发育时间、成虫重量和平均相对生长率,以及不同诱导处理下小麦98-10-30特异基因的表达.结果表明,用针刺、蚜虫取食和BTH(benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester)处理98-10-30后,蚜虫发育成成虫的时间缩短,成虫体重下降,平均相对生长率在各处理间无显著差异.对处理后小麦体内特异基因的表达研究表明,LOX、AOS、PDF1.2、PAL1、PR-1和BGL2基因在mRNA的表达量或质上存在差异.PDF1.2基因在蚜虫取食后mRNA的量显著增加,但BTH处理却不诱导PDF1.2基因的表达,同时,BGL2基因在蚜虫取食和BTH处理后,mRNA的量增加,但对照和针刺处理后BGL2基因不表达.不同诱导处理表明,98-10-30诱导的抗性对麦二叉蚜的生长发育有一定影响,蚜虫取食诱导的防卫反应与机械损伤和BTH处理有相似或相同之处,但存在明显差异.这说明蚜虫诱导的抗性是一种特异反应,与受伤反应和抗病反应有重叠交叉之处,但又不同于二者诱导产生的抗性反应.

关键词: 草莓, 光合特征, 遮荫

Abstract: This paper studied the development duration,adult weight,and mean relative growth rate (MRGR) of aphid Schizapis graminum,and the specific genes expression in wheat variety 98-10-30 (Triticum aestivum) after treated with different elicitors.The results showed that needling penetration,aphid feeding and BTH application could shorten the development duration of the aphid and decrease its adult weight,but had no significant effect on aphid MRGR.Different elicitors induced different specific genes expression in quality and quantity.The mRNA of PDF1.2 was increased significantly after aphid feeding,while there was no expression after applying BTH.Aphid feeding and BTH application increased the mRNA of BGL2,but no expression was observed in the control and after needling penetration.The induced resistance had some effects on aphid growth and development,and the response induced by aphid feeding had some similarities but significant differences to that induced by mechanical wounding and BTH application.It could be concluded that the response of aphid to elicitors was a special resistance,and there existed some overlaps or differences between it and mechanical wounding and SAR (systemic acquired resistance).

Key words: Strawberry, Photosynthesis character, Shading