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汞对土壤酶活性的影响

杨春璐1,2,3;孙铁珩1,2;和文祥1;陈苏2,3   

  1. 1沈阳大学环境工程重点实验室, 沈阳 110044;
    2中国科学院沈阳应用生态研究所, 沈阳 110016;
    3中国科学院研究生院, 北京 100039
  • 收稿日期:2006-02-23 修回日期:2007-01-07 出版日期:2007-03-18 发布日期:2007-03-18

Effects of Hg on soil enzyme activity

YANG Chun-lu1,2,3; SUN Tie-heng1,2; HE Wen-xiang1; CHEN Su2,3   

  1. 1Key Laboratory of Environmental Engineering, Shenyang University, Shenyang 110044, China;
    2Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
    3Graduate University of Chinese Academy of Sciences, Beijing 100039, China
  • Received:2006-02-23 Revised:2007-01-07 Online:2007-03-18 Published:2007-03-18

摘要: 利用室内模拟方法,研究了重金属Hg对不同土样脲酶、转化酶和中性磷酸酶活性的影响.结果表明,Hg可显著地抑制土壤脲酶和转化酶的活性,但不同土样Hg对两种酶活性的抑制程度有很大差别.HgCl2浓度与两种酶活性之间的关系均可用对数方程很好地描述(P<0.05).4个土样的脲酶ED50(生态剂量)分别为87.99、5.47、24.05和19.88 mg·kg-1;转化酶的ED50分别为76.68、727.49、236.52和316.59 mg·kg-1.脲酶对Hg污染比转化酶敏感;有机质对土壤酶活性有一定的保护作用.除连续2年施用大量有机肥的草甸棕壤土样中Hg对中性磷酸酶有显著的激活作用外(P<0.05),其它土样无显著变化,表明中性磷酸酶活性对Hg污染反应不敏感.

关键词: 刈牧强度, 冷蒿, 生长, 资源分配

Abstract: With simulation test, this paper studied the effects of Hg on the activities of urease, in-vertase and neutral phosphotase in four soils. The results showed that Hg inhibited soil urease and invertase activities markedly, but its inhibitory effect differed with test soils. There was a significant logarithmic correlation between the concentration of HgCl2 and the activities of these two enzymes (P<0.05). In test soils, the ED50 of urease activity was 87.99, 5.47, 24.05 and 19.88 mg·kg-1, and that of invertase activity was 76.68, 727.49, 236.52 and 316.59 mg·kg-1, respectively. Urease was more sensitive than invertase to Hg contamination, while organic matter had a protective effect on soil enzymes. Soil neutral phosphatase was not sensitive to Hg contamination, except that it was significantly activated by Hg in the meadow brown soil applied with plenty of organic fertilizer.

Key words: Grazing and clipping intensity, Artemisia frigida, Growth, Resource allocation