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应用生态学报 ›› 2012, Vol. 23 ›› Issue (06): 1721-1727.

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平板混合培养木质纤维素降解菌研究进展

陈耀宁1,2**,赵明杰1,2,曾光明1,2,余震1,2,张嘉超1,2,虞泳1,2,胡春晓1,2   

  1. (1湖南大学环境科学与工程学院, 长沙 410082; 2湖南大学环境生物与控制教育部重点实验室, 长沙 410082)
  • 出版日期:2012-06-18 发布日期:2012-06-18

Research progress on plate mixed culture of lignocellulolytic microorganisms.

CHEN Yao-ning1,2, ZHAO Ming-jie1,2, ZENG Guang-ming1,2, YU Zhen1,2, ZHANG Jia-chao1,2, YU Yong1,2, HU Chun-xiao1,2   

  1. (1College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; 2Ministry of Education Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Changsha 410082, China)
  • Online:2012-06-18 Published:2012-06-18

摘要: 微生物的混合培养已广泛应用于木质纤维素类物质的转化与降解领域.不同木质纤维素降解菌在混合培养时的相互关系在很大程度上影响混合培养的效果.目前对这种相互关系的研究主要依托平板混合培养展开,所用到的平板主要有基础培养基平板和改进培养基平板两种.其中基础培养基平板法主要根据菌落形态、菌丝体颜色、胞外挥发性有机化合物成分和典型胞外酶活性等进行研究,而改进培养基平板则是将基础培养基平板中的碳源更换为天然木质纤维素类物质进行对比研究.本文综述了采用平板混合培养不同木质纤维素降解菌菌株的研究现状和进展,并对该领域研究应重点关注的问题进行了展望.

关键词: 木质纤维素降解菌, 平板, 混合培养, 相互关系

Abstract: Mixed culture of microorganisms has been widely used for the research of lignocellulose transformation and degradation, but the results of the mixed culture are largely affected by the interactions of different lignocellulolytic microorganisms. At present, the researches on these interactions are mainly based on plate mixed culture assay. For this assay, two types of plate were used, namely, basic medium plate and improved medium plate. The basic medium plate is mainly used for the study of colony morphology, mycelia color, exocellular volatile organic compounds, and exocellular enzyme activity, whereas the improved medium plate is used for comparative study, with the carbon sources replaced by natural lignocelloses. This paper summarized the present research status and advancement about the plate mixed culture of lignocellulolytic microorganisms, and put forward a prospect about the focuses of future research in this field.

Key words: lignocellulolytic microorganism, plate, mixed culture, interaction.