Abstract: Bacteriophages infecting rhizobia of legume leads to a significant decrease in the number of rhizobia in soil and nodulation in legume, which finally affects nitrogen fixation and remarkably reduces crop yield. However, limited studies have focused on bacteriophages of rhizobia. In this study, three typic rhizobium strains of Bradyrhizobium diazoefficiens USDA110^T, Sinorhizobium sojae CCBAU05684^T and Sinorhizobium meliloti USDA1002^T were used as the host bacteria. From each host, three rhizobiophages were isolated from an agricultural black soil with double-layer plate method. We isolated nine rhizobiophages and investigated their morphological feature and biological characteristics. The results showed that the phages of SMM (infecting Sinorhizobium meliloti USDA1002^T) and BDM (infecting Bradyrhizobium diazoefficiens USDA110^T) belonged to Myoviridae family, while phages of SSS (infecting Sinorhizobium sojae CCBAU05684^T) belonged to Siphoviridae family. The optimal multiplicity of infection for nine phages ranged from 0.001 to 1.0. The one-step growth curve showed that the latent and rising periods of BDM were remarkably longer than that of SMM and SSS, but the bust size was minimal. Nine phages had the strongest infecting activity at 30-40 ℃ and at neutral pH condition. Comparative analysis showed that the biological characteristics of phages infected with the same host were different, with the differentiation being smaller than that of phages infected with different hosts.