欢迎访问《应用生态学报》官方网站,今天是 分享到:

应用生态学报 ›› 2021, Vol. 32 ›› Issue (3): 887-894.doi: 10.13287/j.1001-9332.202103.039

• 研究论文 • 上一篇    下一篇

云南松林次生演替阶段土壤细菌群落的变化

徐凡迪, 李帅锋, 苏建荣*   

  1. 中国林业科学研究院资源昆虫研究所/国家林业局普洱森林生态系统定位研究站, 昆明 650224
  • 收稿日期:2020-08-26 接受日期:2020-12-16 出版日期:2021-03-15 发布日期:2021-09-15
  • 通讯作者: * E-mail: jianrongsu@vip.sina.com
  • 作者简介:徐凡迪, 女, 1987年生, 硕士研究生。主要从事人工林培育与多功能提升的研究。E-mail: 314982424@qq.com
  • 基金资助:
    中国林业科学研究院中央级公益性科研院所基本科研业务费专项(CAFYBB2020ZA002)和云南省基础研究计划项目面上项目(2019FB058)资助

Changes of soil bacterial community structure at the secondary successional stages in the Pinus yunnanensis forest

XU Fan-di, LI Shuai-feng, SU Jian-rong*   

  1. Research Institute of Resource Insects, Chinese Academy of Forestry/Pu’er Forest Ecosystem Research Station, State Forestry Bureau, Kunming 650224, China
  • Received:2020-08-26 Accepted:2020-12-16 Online:2021-03-15 Published:2021-09-15
  • Contact: * E-mail: jianrongsu@vip.sina.com
  • Supported by:
    Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2020ZA002) and Applied Basic Research Foundation of Yunnan Province (2019FB058)

摘要: 土壤细菌多样性是维持森林生态系统功能的关键因子,森林演替是影响其动态变化的重要因素。研究云南松林不同演替阶段土壤细菌群落结构及其多样性的变化规律,有助于深入理解森林生态系统恢复过程的驱动机制。本研究以云南省永仁县皆伐后形成的针叶林、针阔混交林和常绿阔叶林为对象,基于Illumina Hiseq高通量测序技术,分析森林演替过程中土壤细菌群落组成、结构、多样性及其影响因子的变化。结果表明: 土壤细菌的种群分类单元、Ace指数、Chao1指数和Shannon指数均随着演替进行呈减少趋势,演替早期阶段土壤的细菌总数、菌群丰富度及复杂程度最高。不同演替阶段细菌群落结构存在显著差异,其中,针阔混交林的差异最大,变形菌门和酸杆菌门为各演替序列共有的优势类群,放线菌门、绿弯菌门和Patescibacteria是演替早期的优势类群,且随着演替进行呈现减少趋势;变形菌门和WPS-2相对多度随演替进行呈增加趋势。土壤pH和乔木层物种丰富度是驱动次生演替过程中土壤细菌群落组成变化的关键因子。随着演替的进行,土壤细菌多样性减少,群落组成差异加大。

关键词: 细菌多样性, 群落组成, 高通量测序, 植物多样性, 土壤养分

Abstract: Soil bacterial diversity is a key factor for the maintanence of forest ecosystem function. Soil bacterial community would change along forest succession. We analyzed the variations of soil bacterial diversity and community composition at different successional stages in the Pinus yunnanensis forest, which would help understand the mechanism underlying forest restoration. We investigated soil bacterial diversity, community composition, and effect factors at different successional stages (including coniferous forest, mixed coniferous and broadleaf forest, and evergreen broadleaf forest) using Illumina Hiseq platform. The results showed that OTUs, Chao1 index, Ace index, and Shannon index of soil bacterial community decreased with the process of secondary succession. The highest soil bacterial total OTUs, richness, and complexity appeared at early successional stage. Soil bacterial community composition varied across different stages, with the mixed coniferous and broadleaf forest showing largest variation. Proteobacteria and Acidobacteria were common dominant phyla at secondary successional stages. Actinobacteria, Chloroflexi, and Patescibacteria were dominant phyla at the early successional stage, the abundance of which decreased with successional process in the P. yunnanensis forest. Proteobacteria and WPS-2 increased with the succession. Soil pH and tree species richness were key factors in driving soil bacterial community structure. The soil bacterial diversity decreased with forest succession, while the variations of soil bacterial community composition became larger.

Key words: bacterial diversity, community composition, high-throughput sequencing, plant diversity, soil nutrient