浓硫酸处理对海滨木槿10个家系种子萌发的影响

应用生态学报 ›› 2012, Vol. 23 ›› Issue (11): 2968-2974.

浓硫酸处理对海滨木槿10个家系种子萌发的影响

王小雪^1,2,孙海菁^1**,刘芸²,陈益泰¹,冯大兰³,李沙⁴

(¹中国林业科学研究院亚热带林业研究所, 浙江富阳 311400; ²西南大学资源环境学院, 重庆 400716； ³重庆市林业科学研究院三峡库区森林生态保护与恢复重庆市市级重点实验室, 重庆 400036; ⁴中国民主同盟广西壮族自治区委员会, 南宁 530023)

出版日期:2012-11-18 发布日期:2012-11-18

Effects of treating with concentrated sulfuric acid on the seed germination of ten Hibiscus hamabo provenance families.

WANG Xiao-xue^1,2, SUN Hai-jing¹, LIU Yun²,CHEN Yi-tai¹, FENG Da-lan³, LI Sha⁴

(¹Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China;
²College of Resource and Environment, Southwest China University, Chongqing 400716, China； ³Chongqing Key Laboratory of the Three Gorges Area Forest Ecology Protection and Restoration, Chongqing Academy of Forestry, Chongqing 400036, China;
⁴Guangxi Zhuang Autonomous Region Committee of China Democratic League, Nanning 530023, China)

Online:2012-11-18 Published:2012-11-18

摘要/Abstract

摘要： 以海滨木槿10个家系种子为材料,采用浓硫酸不同时间(0、10、15和20 min)处理,测定种子发芽率、发芽势、发芽指数及萌发过程中相对吸水率、可溶性糖、淀粉和α-淀粉酶含量,研究浓硫酸处理对海滨木槿种子萌发的影响以及不同家系间种子萌发差异.结果表明：浓硫酸处理显著提高了海滨木槿种子的发芽率、发芽指数和发芽势,缩短了发芽时间.浓硫酸处理15 min后,种子发芽率、发芽势和发芽指数分别为95.7%、91.3%和13.28,萌发时间最短,效果最佳.海滨木槿不同家系间种子发芽率、发芽势和发芽指数差异显著,最高发芽指数(15.13)是最低发芽指数(7.15)的2.12倍,最高发芽势(98.0%)是最低发芽势(55.5%)的1.77倍.浓硫酸处理可加快海滨木槿种子萌发代谢生理生化进程.相对吸水率、可溶性糖和α-淀粉酶均呈单峰曲线变化(先升后降),在萌发初期达到最大值；淀粉含量与种子发芽指数呈显著负相关.

Abstract: The seeds from ten Hibiscus hamabo provenance families were treated with concentrated sulfuric acid for different durations (0, 10,
15, and 20 min), and the seed germination rate, germination energy, and germination index, as well as the seed relative water adsorption rate, soluble sugar and starch contents, and α-amylase activity during the germination, were determined, aimed to study the effects of treating with concentrated sulfuric acid on the seed germination of H. hamabo and the differences of the seed germination among different H. hamabo provenance families. After treated with concentrated sulfuric acid, the seed germination rate, germination energy, and germination index increased significantly, and the germination time shortened remarkably. Treating with concentrated sulfuric acid for 15 minutes had the best effect, i.e., the germination rate, germination energy, and germination index were up to 95.7%, 91.3%, and 13.28, respectively, and the germination time was the shortest. The seed germination rate, germination energy, and germination index differed significantly with different provenance families, the highest germination index (15.13) being 2.12 times of the lowest germination index (7.15), and the highest germination energy (98.0%) being 1.77 times of the lowest one (55.5%). Treating with concentrated sulfuric acid accelerated the physiological and biochemical processes of seed germination, and the relative water absorption rate, soluble sugar content, and α-amylase activity were decreased after an initial increase, with the maximum at the early stage of germination. There was a significant negative relationship between the seed starch content and the seed germination index.

王小雪1,2,孙海菁1**,刘芸2,陈益泰1,冯大兰3,李沙4. 浓硫酸处理对海滨木槿10个家系种子萌发的影响[J]. 应用生态学报, 2012, 23(11): 2968-2974.

WANG Xiao-xue1,2, SUN Hai-jing1, LIU Yun2,CHEN Yi-tai1, FENG Da-lan3, LI Sha4. Effects of treating with concentrated sulfuric acid on the seed germination of ten Hibiscus hamabo provenance families.[J]. Chinese Journal of Applied Ecology, 2012, 23(11): 2968-2974.