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NO3-胁迫及恢复对黄瓜幼苗叶片叶绿素荧光参数及ATPase活性的影响

杨凤娟1;王秀峰1;魏珉1;苏秀荣2;闫童1   

  1. 1山东农业大学园艺科学与工程学院,泰安 271018;2山东农业大学化学与材料科学学院,泰安 271018

  • 收稿日期:2005-04-01 修回日期:2005-07-11 出版日期:2006-03-18 发布日期:2006-03-18

Effects of NO3-stress and recovery on chlorophyll fluorescence parameters and ATPase activities of cucumber seedling leaves

YANG Fengjuan1;WANG Xiufeng1;WEI Min1;SU Xiurong2;YAN Tong1   

  1. 1College of Horticulture Science and Engineering,Shandong Agricultural Univeristy,Tai'an 271018,China;2College of Chemistry and Material Science,Shandong Agricultural University,Tai’an 271018,China

  • Received:2005-04-01 Revised:2005-07-11 Online:2006-03-18 Published:2006-03-18

摘要: 通过水培试验,探讨了不同NO3-浓度胁迫及恢复对黄瓜幼苗叶片叶绿素含量、叶绿素荧光参数及ATPase活性的影响.结果表明,胁迫7 d后,高浓度NO3-(168 mmol·L-1)可极显著提高叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量,极显著提高初始荧光(Fo)、MgATPase和Ca-ATPase活性,而PSⅡ原初光能转化效率(Fv/Fm)、PSⅡ潜在活性(Fv/Fo)和PSⅡ光合电子传递量子效率(ΦPSⅡ),却随NO3-浓度的增加而降低.恢复7 d后,所有处理叶绿素和类胡萝卜素含量均低于对照;初始荧光基本都恢复至对照水平;PSⅡ原初光能转化效率和PSⅡ光合电子传递量子效率在NO3-浓度低于126 mmol·L-1时,基本恢复至对照水平,而高于这一水平时,仍显著低于对照;PSⅡ潜在活性在NO3-浓度为42和126 mmol·L-1的处理基本达对照水平,其它处理仍极显著低于对照;Mg-ATPase和Ca-ATPase活性均出现先降低后升高的变化趋势.

关键词: 雪被, 全球变暖, 土壤温度, 土壤养分, 川西高山森林

Abstract: With water culture,this paper studied the effects of NO3- stress and recovery on the chlorophyll contents,chlorophyll fluorescence parameters,and ATPase activities of cucumber seedling leaves.The results showed that under stress for 7 days,the chlorophyll a,chlorophyll b,total chlorophyll and carotenoid contents,intrinsic fluorescence (Fo),and MgATPase and Ca-ATPase activities were increased significantly when the NO3- concentration was high (168 mmol ·L-1),while the intrinsic photochemical efficiency (Fv/Fm),potential activities (Fv/Fo) and quantum yield (ΦPSⅡ) of PSⅡ were decreased with increasing NO3- concentration.After recovery for 7 days,the chlorophyll and carotenoid contents of all treatments with NO3- were lower than CK,while the intrinsic fluorescence,intrinsic photochemical efficiency and quantum yield of PSⅡ were similar to CK when the NO3- concentration was ≤ 84 mmol·L-1,and the Fv/Fm and ΦPSⅡ were significantly lower than CK when the NO3- concentration was>84 mmol·L-1.The potential activity of PSⅡ was similar to CK when the NO3- concentration was 42 and 126 mmol·L-1,but significantly lower than CK in other treatments.The activities of Mg-ATPase and Ca-ATPase were decreased firstly but increased then when the NO3- concentration was>84 mmol·L-1.

Key words: snow pack, global warming, soil temperature, soil nutrient, alpine forests of west Sichuan