不同比例盐生小球藻-枯草芽孢杆菌共生体对砷酸盐耐性及富集差异

doi:10.13287/j.1001-9332.202010.032

应用生态学报 ›› 2020, Vol. 31 ›› Issue (10): 3539-3546.doi: 10.13287/j.1001-9332.202010.032

不同比例盐生小球藻-枯草芽孢杆菌共生体对砷酸盐耐性及富集差异

于清男¹, 陈双双¹, 郑超¹, 张春华², 葛滢^1*

¹南京农业大学资源与环境科学学院, 江苏省海洋生物学重点实验室, 南京 210095;
²南京农业大学生命科学实验中心, 元素与生命科学示范实验室, 南京 210095

收稿日期:2020-04-07 接受日期:2020-07-02 出版日期:2020-10-15 发布日期:2021-04-15
通讯作者: * E-mail: yingge711@njau.edu.cn
作者简介:于清男, 女, 1996年生, 硕士研究生。主要从事水体重金属生物修复研究。E-mail: 2017103001@njau.edu.cn
基金资助:
国家自然科学基金项目(31770548,31400450)资助

Differences in the tolerance and accumulation of arsenate in the consortia with various proportions of Chlorella salina and Bacillus subtilis

YU Qing-nan¹, CHEN Shuang-shuang¹, ZHENG Chao¹, ZHANG Chun-hua², GE Ying^1*

¹Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China;
²Laboratory Centre of Life Science, Demonstration Laboratory of Elements and Life Science, Nanjing Agricultural University, Nanjing 210095, China

Received:2020-04-07 Accepted:2020-07-02 Online:2020-10-15 Published:2021-04-15
Contact: * E-mail: yingge711@njau.edu.cn
Supported by:
National Natural Science Foundation of China (31770548, 31400450).

摘要/Abstract

摘要： 采用盐生小球藻与枯草芽孢杆菌为供试材料,构建不同藻菌比(1∶0、1∶1、1∶2、1∶3、1∶4)的共生体,在不同浓度砷酸盐[As(Ⅴ)]处理7 d后,测定藻菌共生体生长及其对砷的富集、吸附、吸收和形态转化。结果表明: 在As(Ⅴ)处理下,随着枯草芽孢杆菌比例的增加,藻菌共生体叶绿素含量、干重、比增长率显著提高,在750 μg·L^-1As(Ⅴ)处理时,1∶4的藻菌共生体分别达到1.81 mg·L^-¹、125.0 mg、0.28 mg·L^-¹·d^-¹。藻菌比例由1∶0变为1∶4时,藻菌共生体对砷的富集和吸收呈下降趋势;砷的富集方式随砷浓度的增加而变化,在75～150 μg·L^-1As(Ⅴ)处理下以吸收为主,在300～750 μg·L^-1 As(Ⅴ)处理下以吸附为主。藻菌共生体内存在As(Ⅴ)和As(Ⅲ)两种形态,且随枯草芽孢杆菌比例的上升,As(Ⅴ)还原率增大(最高达到12.6%)。综上,枯草芽孢杆菌的添加提升了藻菌共生体对As(Ⅴ)的耐性和还原,但减少了对As(Ⅴ)的富集。

关键词: 藻菌共生体, 砷酸盐, 生长, 富集, 形态转化

Abstract: We constructed consortia of Chlorella salina and Bacillus subtilis with various alga-bacterium ratios (1:0, 1:1, 1:2, 1:3, 1:4). After being treated with arsenate [As(Ⅴ)] for 7 d, we measured the growth, As accumulation, adsorption and absorption, and As speciation transformation of consortia. Results showed that the chlorophyll content, dry weight, and specific growth rate of the symbiont increased significantly with increasing B. subtilis ratio after the As(Ⅴ) treatment, being 1.81 mg·L^-1, 125.0 mg, and 0.28 mg·L^-1·d^-1 under the condition of the alga-bacterium ratio being 1∶4 and As(Ⅴ) being 750 μg·L^-1, respectively. The accumulation and absorption of As by the consortia decreased with the bacterial proportion increasing from 1:0 to 1:4. As accumulation changed with the As concentration, with a dominance of absorption under 75-150 μg·L^-1 As(Ⅴ) and a dominance of adsorption under 300-750 μg·L^-1As(V). There were As(Ⅴ) and As(Ⅲ) in the consortia. When the proportion of bacteria increased, the rate of As(Ⅴ) reduction enhanced (up to 12.6%). Our results indicated that the increases of B. subtilis improved As(Ⅴ) tolerance and reduction, but decreased the As(Ⅴ) accumulation by the symbiont.

Key words: algae-bacteria consortia, arsenate, growth, accumulation, speciation transformation

于清男, 陈双双, 郑超, 张春华, 葛滢. 不同比例盐生小球藻-枯草芽孢杆菌共生体对砷酸盐耐性及富集差异[J]. 应用生态学报, 2020, 31(10): 3539-3546.

YU Qing-nan, CHEN Shuang-shuang, ZHENG Chao, ZHANG Chun-hua, GE Ying. Differences in the tolerance and accumulation of arsenate in the consortia with various proportions of Chlorella salina and Bacillus subtilis[J]. Chinese Journal of Applied Ecology, 2020, 31(10): 3539-3546.

参考文献

[1] 刘艳丽, 徐莹, 杜克兵, 等. 无机砷在植物体内的吸收和代谢机制. 应用生态学报, 2012, 23(3): 842-848 [Liu Y-L, Xu Y, Du K-B, et al. Absorption and metabolism mechanisms of inorganic arsenic in plants: A review. Chinese Journal of Applied Ecology, 2012, 23(3): 842-848]
[2] Ma Z, Lin L, Wu M, et al. Total and inorganic arsenic contents in seaweeds: Absorption, accumulation, transformation and toxicity. Aquaculture, 2018, 497: 49-55
[3] Cho DH, Ramanan R, Heo J, et al. Enhancing microalgal biomass productivity by engineering a microalgal-bacterial community. Bioresource Technology, 2015, 175: 578-585
[4] Ji X, Jiang M, Zhang J, et al. The interactions of algae-bacteria symbiotic system and its effects on nutrients removal from synthetic wastewater. Bioresource Techno-logy, 2018, 247: 44-50
[5] Hom EFY, Aiyar P, Schaeme D, et al. A chemical perspective on microalgal-microbial interactions. Trends in Plant Science, 2015, 20: 689-693
[6] 左新宇. 微囊藻与硝化细菌相互关系的初步研究. 硕士论文. 武汉: 华中农业大学, 2013 [Zuo X-Y. Preliminary Studies on Relationship between Microcystis sp. and Nitrifying Bacteria. Master Thesis. Wuhan: Huazhong Agricultural University, 2013]
[7] Majeed A, Muhammad Z, Ahmad H. Plant growth promoting bacteria: Role in soil improvement, abiotic and biotic stress management of crops. Plant Cell Reports, 2018, 37: 1599-1609
[8] 霍伟, 蔡庆生. 植物促生菌提高植物重金属耐受性研究进展. 微生物学通报, 2010, 37(9): 1374-1378 [Huo W, Cai Q-S. The advance of enhancement of plant heavy metal resistance by plant growth-promote bacteria. Microbiology China, 2010, 37(9): 1374-1378]
[9] 胡江春, 薛德林, 马成新, 等. 植物根际促生菌(PGPR)的研究与应用前景. 应用生态学报, 2004, 15(10): 1963-1966 [Hu J-C, Xue D-L, Ma C-X, et al. Research advances in plant growth promoting rhizobacteria and its application prospects. Chinese Journal of Applied Ecology, 2004, 15(10): 1963-1966]
[10] Amavizca E, Bashan Y, Ryu CM, et al. Enhanced performance of the microalga Chlorella sorokiniana remotely induced by the plant growth-promoting bacteria Azospirillum brasilense and Bacillus pumilus. Scientific Reports, 2017, 7: 41310-41321
[11] Burd GI, Dixon DG, Glick BR. Plant growth-promoting bacteria that decrease heavy metal toxicity in plants. Canadian Journal of Microbiology, 2000, 46: 237-245
[12] El-Meihy RM, Abou-Aly HE, Youssef AM, et al. Efficiency of heavy metals-tolerant plant growth promoting bacteria for alleviating heavy metals toxicity on sorghum. Environmental and Experimental Botany, 2019, 162: 295-301
[13] 王亚, 张春华, 王淑, 等. 带菌盐藻对不同形态砷的富集和转化研究. 环境科学, 2013, 34(11): 4257-4265 [Wang Y, Zhang C-H, Wang S, et al. Accumulation and transformation of different arsenic species in nonaxenic Dunaliella salina. Environmental Science, 2013, 34(11): 4257-4265]
[14] 许平平, 刘聪, 王亚, 等. 共生细菌对盐生小球藻富集和转化砷酸盐的影响. 环境科学, 2016, 37(9): 3438-3446 [Xu P-P, Liu C, Wang Y, et al. Effects of a symbiotic bacterium on the accumulation and transformation of arsenate by Chlorella salina. Environmental Science, 2016, 37(9): 3438-3446]
[15] 王亚茹, 潘晓, 于清男, 等. 共生细菌对盐生小球藻亚砷酸盐代谢的影响. 中国环境科学, 2019, 39(10): 4303-4312 [Wang Y-R, Pan X, Yu Q-N, et al. Effect of a symbiotic bacterium on the accumulation and transformation of arsenite by Chlorella salina. China Environmental Science, 2019, 39(10): 4303-4312]
[16] 王华光, 王文静, 盛彦清. 污水处理工艺对微藻及藻际细菌群落的影响. 中国环境科学, 2018, 38(10): 3761-3766 [Wang G-H, Wang W-J, Sheng Y-Q. Influences of sewage treatments technologies on microalgae and bacteria community. China Environmental Science, 2018, 38(10): 3761-3766]
[17] 李林, 朱伟. 不同光照条件下水流对铜绿微囊藻生长的影响. 湖南大学学报: 自然科学版, 2012, 39(9): 87-92 [Li L, Liu W. Effects of water flow under diffe-rent light intensity on the growth of Microcystis aerugi-nosa. Journal of Hunan University: Natural Sciences, 2012, 39(9): 87-92]
[18] Ji X, Li H, Zhang J, et al. The collaborative effect of Chlorella vulgaris - Bacillus licheniformis consortia on the treatment of municipal water. Journal of Hazardous Materials, 2019, 365: 483-493
[19] 张晶, 侯和胜, 佟少明. 微藻与细菌作用关系的研究进展. 激光生物学报, 2016, 25(5): 385-390 [Zhang J, Hou H-S, Tong S-M. Research progress of interaction between microalgae and bacteria. Acta Laser Biology Sinica, 2016, 25(5): 385-390]
[20] Palacios OA, Gomez-Anduro G, Bashan Y, et al. Tryptophan, thiamine and indole-3-acetic acid exchange between Chlorella sorokiniana and the plant growth-promoting bacterium Azospirillum brasilense. FEMS Micro-biology Ecology, 2016, 92: fiw077
[21] 马莹, 骆永明, 滕应, 等. 根际促生菌及其在污染土壤植物修复中的应用. 土壤学报, 2013, 50(5): 1021-1031 [Ma Y, Luo Y-M, Teng Y, et al. Plant growth promoting rhizobacteria and their role in phytoremediation of heavy metal contaminated soils. Acta Pedologica Sinica, 2013, 50(5): 1021-1031]
[22] 阮迪申, 曾加会, 晁元卿, 等. 重金属胁迫下内生菌对宿主植物的解毒机制. 微生物学通报, 2016, 43 (12): 2700-2706 [Ruan D-S, Zeng J-H, Chao Y-Q, et al. Role of endophytes in plant tolerance to heavy metal stress. Microbiology China, 2016, 43(12): 2700-2706]
[23] 彭少麟, 杜卫兵, 李志安. 不同生态型植物对重金属的积累及耐性研究进展. 吉首大学学报:自然科学版, 2004, 25(4): 19-26 [Peng S-L, Du W-B, Li Z-A. A review of heavy metal accumulation and tolerance by plants of different ecotype. Journal of Jishou Univer-sity: Natural Sciences, 2004, 25(4): 19-26]
[24] Cullen WR, Li H, Hewitt G, et al. Identification of extracellular arsenical metabolites in the growth medium of the microorganisms Apiotrichum humicola and Scopulariopsis brevicaulis. Applied Organometallic Chemistry, 1994, 8: 303-311
[25] Levy JL, Stauber JL, Adams MS, et al. Toxicity, biotransformation, and mode of action of arsenic in two freshwater microalgae (Chlorella sp. and Monoraphidium arcuatum). Environmental Toxicology and Chemistry, 2005, 24: 2630-2639
[26] Wang Z, Luo Z, Yan C. Accumulation, transformation, and release of inorganic arsenic by the freshwater cyanobacterium Microcystis aeruginosa. Environmental Science and Pollution Research, 2013, 20: 7286-7295
[27] 李景龙, 孙玉青, 陈保冬, 等. 丛枝菌根真菌砷酸盐还原酶基因 RiarsC 的克隆和功能分析. 生态毒理学报, 2018, 13(3): 71-77 [Li J-L, Sun Y-Q, Chen B-D, et al. Cloning and characterization of arsenate reductase gene RiarsC from arbuscular mycorrhizal fungi. Asian Journal of Ecotoxicology, 2018, 13(3): 71-77]
[28] Pickering IJ, Prince RC, George MJ, et al. Reduction and coordination of arsenic in Indian mustard. Plant Physiology, 2000, 122: 1171-1178
[29] Lombi E, Zhao FJ, Fuhrmann M, et al. Arsenic distribution and speciation in the fronds of the hyperaccumulator Pteris vittata. New Phytologist, 2002, 156: 195-203
[30] Guo P, Gong Y, Wang C, et al. Arsenic speciation and effect of arsenate inhibition in a Microcystis aeruginosa culture medium under different phosphate regimes. Environmental Toxicology and Chemistry, 2011, 30: 1754-1759
[31] Bahar MM, Megharaj M, Naidu R. Influence of phosphate on toxicity and bioaccumulation of arsenic in a soil isolate of microalga Chlorella sp. Environmental Science and Pollution Research, 2016, 23: 2663-2668
[32] Baker J, Wallschläger D. The role of phosphorus in the metabolism of arsenate by a freshwater green alga, Chlorella vulgaris. Journal of Environmental Sciences, 2016, 49: 169-178
[33] Wang NX, Li Y, Deng XH, et al. Toxicity and bioaccumulation kinetics of arsenate in two freshwater green algae under different phosphate regimes. Water Research, 2013, 47: 2497-2506
[34] 王静. 铜绿微囊藻中砷的代谢与生物效应. 硕士论文. 天津: 天津大学, 2012 [Wang J. Metabolism and Biological Effects of Arsenic in Microcystis aeruginosa. Master Thesis. Tianjing: Tianjing University, 2012]
[35] Duncan EG, Maher WA, Foster SD, et al. The influence of arsenate and phosphate exposure on arsenic uptake, metabolism and species formation in the marine phytoplankton Dunaliella tertiolecta. Marine Chemistry, 2013, 157: 78-85
[36] Zhang S, Rensing C, Zhu YG. Cyanobacteria-mediated arsenic redox dynamics is regulated by phosphate in aquatic environments. Environmental Science and Technology, 2014, 48: 994-1000

不同比例盐生小球藻-枯草芽孢杆菌共生体对砷酸盐耐性及富集差异

Differences in the tolerance and accumulation of arsenate in the consortia with various proportions of Chlorella salina and Bacillus subtilis

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