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应用生态学报 ›› 2002, Vol. ›› Issue (10): 1283-1286.

• 研究论文 • 上一篇    下一篇

浙江产车前(Plantago asiatica)种群遗传分化的主坐标分析

郭水良1, 张东旭1, 曹同2   

  1. 1. 浙江师范大学生命与环境科学学院, 金华, 321004;
    2. 中国科学院沈阳应用生态研究所, 沈阳, 110016
  • 收稿日期:2001-08-23 修回日期:2002-02-17 出版日期:2002-10-15
  • 通讯作者: 郭水良,男,1964年生,博士后,研究员,从事植物分类与生态学研究,发表论文60余篇.E-mail:guoshuiliang@163.com
  • 基金资助:
    国家教育部高等学校骨干教师资助计划项目(No.1821).

GUO Shuiliang1, ZHANG Dongxu1, CAO Tong2   

  1. 1. College of Life and Environment Sciences, Zhejiang Normal University, Jinhua, 321004;
    2. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016
  • Received:2001-08-23 Revised:2002-02-17 Online:2002-10-15

摘要: 运用18个有效的10mer-寡核苷酸引物对浙江产车前(Plantago asiatica)的8个种群基因组DNA进行了PCR扩增,共检测到186个位点,其中多态位点125个,占68.70%.应用Jaccard公式计算了8个种群在125个位点上的相似性.以此为基础,应用主坐标排序,作出了8个种群遗传分化的三维排序图.结果表明,8个种群的遗传分化与地理位置、海拔高度有联系.

关键词: 车前, 种群, 随机扩增多态性DNA, 主坐标分析

Abstract: Eight populations of Plantago asiatica collected from Zhejiang were analyzed using random amplified polymorphic DNA (RAPD). Eighteen of the 60 random primers were selected.186 bands were amplified by the 18 primers,and 68.70% of them were polymorphic. Jaccard coefficient was applied to compare the genetic of these eight populations based on the RAPDdata. Three-dimensional ordination plot of the eight populations based on the Jaccard coefficient was constructed by using principal axes analysis (PAA). The results showed that the genetic differentiation of eight populations could be explained to a certain extent by their geographical location and altitude.

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