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应用生态学报 ›› 2011, Vol. 22 ›› Issue (08): 2179-2183.

• 研究报告 • 上一篇    下一篇

紫外线辐射对西伯利亚鲟精子活力和寿命的影响

张涛1,颜世伟1,2,章龙珍1**,庄平1,2,田美平3,闫文罡1,江淇4,姚志峰3   

  1. 1中国水产科学研究院东海水产研究所农业部海洋与河口渔业资源及生态重点开放实验室, 上海 200090;2大连水产学院生命科学与技术学院, 辽宁大连116023;3上海海洋大学水产与生命学院, 上海 201306;4杭州千岛湖鲟龙科技开发有限公司, 杭州 330127
  • 出版日期:2011-08-18 发布日期:2011-08-18

Impacts of ultraviolet irradiation on the sperm motility and longevity of Acipenser baerii.

ZHANG Tao1, YAN Shi-wei1,2, ZHANG Long-zhen1, ZHUANG Ping1,2, TIAN Mei-ping3, YAN Wen-gang1, JIANG Qi4, YAO Zhi-feng3   

  1. 1Ministry of Agriculture Key and Open Laboratory of Marine and Estuarine Fisheries Resources and Ecology, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China; 2College of Life Science and Technology, Dalian Fisheries University, Dalian 116023, Liaoning, China; 3College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China; 4Hangzhou Qiandaohu Xunlong Technology Development Co. Ltd., Hangzhou 330127, China
  • Online:2011-08-18 Published:2011-08-18

摘要: 研究了不同剂量紫外线辐射(254 nm,UVC)对西伯利亚鲟精子活力和寿命的影响.结果表明:紫外线辐射对精子的活力、快速运动时间和寿命均具有显著性影响.其中,精子活力随辐射剂量的增加而呈先迅速下降,后迅速上升,再迅速下降的趋势;精子快速运动时间的变化趋势与活力相似;精子寿命随辐射剂量的增加呈缓慢下降的趋势.当辐射剂量达288 mJ·cm-2时,精子无快速运动,当辐射剂量达324 mJ·cm-2时,精子活力和寿命均降为0.根据Hertwig效应判断,辐射剂量216 mJ·cm-2为西伯利亚鲟精子灭活的最适剂量.

关键词: 西伯利亚鲟, 精子, 紫外线辐射, 活力, 寿命

Abstract: This paper studied the impacts of different dose ultraviolet irradiation (254 nm, UVC) on the sperm motility and longevity of Acipenser baerii. Ultraviolet irradiation had significant impacts on the sperm motility, its fast motion
 time, and longevity. With the increasing dose of ultraviolet irradiation, the s
perm motility decreased rapidly first, increased rapidly then, and decreased rapidly again. The sperm fast motion time had the similar variation trend as the spe
rm motility, but the sperm longevity kept decreasing with increasing dose of ult
raviolet irradiation. When the ultraviolet irradiation dose increased to 288 mJ·
cm-2, the sperm fast motion disappeared; when the ultraviolet irradiation
dose increased up to 324 mJ·cm-2, the sperm had no motility and died. According to the “Hertwig effect”, the optimum ultraviolet irradiation dose for inactivating A. baerii sperm was 216 mJ·cm-2.

Key words: Acipenser baerii, sperm, ultraviolet irradiation, motility, longevityser