黄山花楸种群遗传多样性研究

应用生态学报 ›› 2003, Vol. ›› Issue (12): 2141-2144.

黄山花楸种群遗传多样性研究

刘登义, 沈浩, 杨月红, 张杰

安徽师范大学生物多样性研究中心, 重要生物资源保护与利用研究安徽省重点实验室, 芜湖 241000

收稿日期:2003-04-16 修回日期:2003-09-15
通讯作者: 刘登义,男,1958年9月生,博士,教授,博导;主要从事植物种群生态学、生物多样性与保护生态学和环境生态学研究,发表论文60余篇,出版专著两部.Tel:0553-3869233,E-mail:ldy@mail.ahnu.edu.cn.
基金资助:
教育部科学技术重点研究项目;重要生物资源保护与利用研究安徽省重点实验室专项基金资助项目

Genetic diversity of rare and endangered plant Sorbus amabilis

LIU Dengyi, SHEN Hao, YANG Yuehong, ZHANG Jie

Biodiversity Research Center, The Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources in Anhui, Anhui Normal University, Wuhu 241000, China

Received:2003-04-16 Revised:2003-09-15

摘要/Abstract

摘要： 利用RAPD技术,对黄山花楸(Sorbus amabilis)自然分布区的13个自然种群的遗传多样性进行了研究.从40个10碱基随机引物中筛选出能产生稳定多态性标记的引物14个,共扩增出105个位点,其中多态性位点30个,占28.6%.应用UPGMA法和Neighbor-Joining法对遗传距离进行聚类分析构建树系图,结果表明,黄山花楸自然种群具有较低的遗传多样性,对环境变化的适应能力较差;其种群间的遗传差异与其地理分布有关;黄山花楸自身的特殊进化历史和人为砍伐以及自然灾害(火灾、病虫害等)和小种群的遗传漂变作用是黄山花楸遗传多样性水平低的主要原因,也是其濒危的主要原因.

关键词: 黄山花楸, 遗传多样性, RAPD

Abstract: The genetic diversity of 13 natural populations of Sorbus amabilis Cheng ex Y? from different distribution regions was investigated using RAPD.Fourteen primers were screened from 40 ten bp arbitrary primers,and a total of 105 DNA fragments were amplified,among which,30(28.6%) were polymorphic. DNA molecular dendrograms were established for the 13 natural populations,based on UPGMA and Neighbor Joining.The result showed that S.amabilis had a low genetic diversity,and its adaptability to environmental change was weak.The genetic diversity of S.amabilis among the 13 natural populations were primarily related to their geographic distribution.Its special evolutionary history,man made destruction,natural disaster (fire,plant diseases and insect pests etc.) and genetic drift caused by small population were the main reasons for the low level genetic diversity of S.amabilis and its endangered position.

Key words: Sorbus amabilis, Genetic diversity, RAPD

中图分类号:

Q943

刘登义, 沈浩, 杨月红, 张杰. 黄山花楸种群遗传多样性研究[J]. 应用生态学报, 2003, (12): 2141-2144.

LIU Dengyi, SHEN Hao, YANG Yuehong, ZHANG Jie . Genetic diversity of rare and endangered plant Sorbus amabilis[J]. Chinese Journal of Applied Ecology, 2003, (12): 2141-2144.

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